LOCUS CAB5247894.1 279 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE LYSOPHOSPHOLIPASE protein. ACCESSION LT708304-193 PROTEIN_ID CAB5247894.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0189" /note="Mb0189, -, len: 279 aa. Equivalent to Rv0183, len: 279 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 279 aa overlap). Possible lysophospholipase (EC 3.1.-.-), similar to several (especially eukaryotic enzymes, weaker with Escherichia coli), e.g. U67963|HSU67963_1 Human lysophospholipase homolog from Homo sapiens (313 aa), FASTA scores: opt: 569, E(): 2.6e-29, (37.1% identity in 259 aa overlap); P07000|PLDB_ECOLI LYSOPHOSPHOLIPASE L2 from Escherichia coli (165 aa), FASTA scores: opt: 219, E(): 0.00012. Start changed based on similarity to AE001997_8 from Deinococcus radiodurans (282 aa), FASTA scores: opt: 510, E(): 1.4e-25, (34.8% identity in 282 aa overlap). Also shows some similarity to epoxide hydrolases from Mycobacterium tuberculosis e.g. Rv1938 FASTA score: (30.7% identity in 114 aa overlap); and O07214|YR15_MYCTU|Rv2715|MT2788|MTCY05A6.36 (341 aa). Note that the putative product of this CDS corresponds to spot 3_329 identified in culture supernatant by proteomics at the Max-Planck-Institut fuer Infektionsbiologie (see citations below). Protein product from Mb0189 detected using shotgun mass spectrometry. Mb0189 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTTTRTERNF AGIGDVRIVY DVWTPDTAPQ AVVVLAHGLG EHARRYDHVA QRLGAAGLVT 61 YALDHRGHGR SGGKRVLVRD ISEYTADFDT LVGIATREYP GCKRIVLGHS MGGGIVFAYG 121 VERPDNYDLM VLSAPAVAAQ DLVSPVVAVA AKLLGVVVPG LPVQELDFTA ISRDPEVVQA 181 YNTDPLVHHG RVPAGIGRAL LQVGETMPRR APALTAPLLV LHGTDDRLIP IEGSRRLVEC 241 VGSADVQLKE YPGLYHEVFN EPERNQVLDD VVAWLTERL //