LOCUS       CAB5247894.1             279 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            LYSOPHOSPHOLIPASE protein.
ACCESSION   LT708304-193
PROTEIN_ID  CAB5247894.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /locus_tag="BQ2027_MB0189"
                     /note="Mb0189, -, len: 279 aa. Equivalent to Rv0183, len:
                     279 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 279 aa overlap). Possible
                     lysophospholipase (EC 3.1.-.-), similar to several
                     (especially eukaryotic enzymes, weaker with Escherichia
                     coli), e.g. U67963|HSU67963_1 Human lysophospholipase
                     homolog from Homo sapiens (313 aa), FASTA scores: opt:
                     569, E(): 2.6e-29, (37.1% identity in 259 aa overlap);
                     P07000|PLDB_ECOLI LYSOPHOSPHOLIPASE L2 from Escherichia
                     coli (165 aa), FASTA scores: opt: 219, E(): 0.00012. Start
                     changed based on similarity to AE001997_8 from Deinococcus
                     radiodurans (282 aa), FASTA scores: opt: 510, E():
                     1.4e-25, (34.8% identity in 282 aa overlap). Also shows
                     some similarity to epoxide hydrolases from Mycobacterium
                     tuberculosis e.g. Rv1938 FASTA score: (30.7% identity in
                     114 aa overlap); and
                     O07214|YR15_MYCTU|Rv2715|MT2788|MTCY05A6.36 (341 aa). Note
                     that the putative product of this CDS corresponds to spot
                     3_329 identified in culture supernatant by proteomics at
                     the Max-Planck-Institut fuer Infektionsbiologie (see
                     citations below). Protein product from Mb0189 detected
                     using shotgun mass spectrometry. Mb0189 found to be
                     expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MTTTRTERNF AGIGDVRIVY DVWTPDTAPQ AVVVLAHGLG EHARRYDHVA QRLGAAGLVT
       61 YALDHRGHGR SGGKRVLVRD ISEYTADFDT LVGIATREYP GCKRIVLGHS MGGGIVFAYG
      121 VERPDNYDLM VLSAPAVAAQ DLVSPVVAVA AKLLGVVVPG LPVQELDFTA ISRDPEVVQA
      181 YNTDPLVHHG RVPAGIGRAL LQVGETMPRR APALTAPLLV LHGTDDRLIP IEGSRRLVEC
      241 VGSADVQLKE YPGLYHEVFN EPERNQVLDD VVAWLTERL
//