LOCUS CAB5247893.1 370 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ALTERNATIVE RNA POLYMERASE SIGMA FACTOR SIGG (RNA POLYMERASE ECF TYPE SIGMA FACTOR) protein. ACCESSION LT708304-192 PROTEIN_ID CAB5247893.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="sigG" /locus_tag="BQ2027_MB0188C" /note="Mb0188c, sigG, len: 370 aa. Equivalent to Rv0182c, len: 370 aa (start site uncertain; first of several possibles was chosen, but note that this overlaps the upstream ORF), from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 370 aa overlap). Probable sigG, alternative RNA polymerase sigma subunit (see citations below), similar to many e.g. Q45585|SIGW_BACSU RNA POLYMERASE SIGMA FACTOR from Bacillus subtilis (187 aa). Also similar to nine other ECF sigma factors from Mycobacterium tuberculosis e.g. Rv1221, Rv0735, etc. Contains PS01063 Sigma-70 factors ECF subfamily signature and probable helix-turn helix motif from aa 205-226 (Score 1181, +3.21 SD). BELONGS TO THE SIGMA-70 FACTOR FAMILY, ECF SUBFAMILY. Protein product from Mb0188c detected using SWATH mass spectrometry. Mb0188c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MRTSPMPAKF RSVRVVVITG SVTAAPVRVS ETLRRLIDVS VLAENSGREP ADERRGDFSA 61 HTEPYRRELL AHCYRMTGSL HDAEDLVQET LLRAWKAYEG FAGKSSLRTW LHRIATNTCL 121 TALEGRRRRP LPTGLGRPSA DPSGELVERR EVSWLEPLPD VTDDPADPST IVGNRESVRL 181 AFVAALQHLS PRQRAVLLLR DVLQWKSAEV ADAIGTSTVA VNSLLQRARS QLQTVRPSAA 241 DRLSAPDSPE AQDLLARYIA AFEAYDIDRL VELFTAEAIW EMPPYTGWYQ GAQAIVTLIH 301 QQCPAYSPGD MRLISLIANG QPAAAMYMRA GDVHLPFQLH VLDMAADRVS HVVAFLDTTL 361 FPKFGLPDSL //