LOCUS       CAB5247893.1             370 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ALTERNATIVE RNA POLYMERASE SIGMA FACTOR SIGG (RNA POLYMERASE
            ECF TYPE SIGMA FACTOR) protein.
ACCESSION   LT708304-192
PROTEIN_ID  CAB5247893.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="sigG"
                     /locus_tag="BQ2027_MB0188C"
                     /note="Mb0188c, sigG, len: 370 aa. Equivalent to Rv0182c,
                     len: 370 aa (start site uncertain; first of several
                     possibles was chosen, but note that this overlaps the
                     upstream ORF), from Mycobacterium tuberculosis strain
                     H37Rv, (100.0% identity in 370 aa overlap). Probable sigG,
                     alternative RNA polymerase sigma subunit (see citations
                     below), similar to many e.g. Q45585|SIGW_BACSU RNA
                     POLYMERASE SIGMA FACTOR from Bacillus subtilis (187 aa).
                     Also similar to nine other ECF sigma factors from
                     Mycobacterium tuberculosis e.g. Rv1221, Rv0735, etc.
                     Contains PS01063 Sigma-70 factors ECF subfamily signature
                     and probable helix-turn helix motif from aa 205-226 (Score
                     1181, +3.21 SD). BELONGS TO THE SIGMA-70 FACTOR FAMILY,
                     ECF SUBFAMILY. Protein product from Mb0188c detected using
                     SWATH mass spectrometry. Mb0188c found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MRTSPMPAKF RSVRVVVITG SVTAAPVRVS ETLRRLIDVS VLAENSGREP ADERRGDFSA
       61 HTEPYRRELL AHCYRMTGSL HDAEDLVQET LLRAWKAYEG FAGKSSLRTW LHRIATNTCL
      121 TALEGRRRRP LPTGLGRPSA DPSGELVERR EVSWLEPLPD VTDDPADPST IVGNRESVRL
      181 AFVAALQHLS PRQRAVLLLR DVLQWKSAEV ADAIGTSTVA VNSLLQRARS QLQTVRPSAA
      241 DRLSAPDSPE AQDLLARYIA AFEAYDIDRL VELFTAEAIW EMPPYTGWYQ GAQAIVTLIH
      301 QQCPAYSPGD MRLISLIANG QPAAAMYMRA GDVHLPFQLH VLDMAADRVS HVVAFLDTTL
      361 FPKFGLPDSL
//