LOCUS CAB5247887.1 322 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED MCE ASSOCIATED TRANSMEMBRANE PROTEIN protein. ACCESSION LT708304-186 PROTEIN_ID CAB5247887.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0182" /note="Mb0182, -, len: 322 aa. Equivalent to Rv0176, len: 322 aa, from Mycobacterium tuberculosis strain H37Rv, (99.4% identity in 322 aa overlap). Probable conserved Mce-associated transmembrane protein. Contains short region of similarity to PRA_MYCLE|P41484 proline-rich antigen (36 kDa antigen) from Mycobacterium leprae (249 aa) (outside the proline-rich region), FASTA scores: opt: 165, E(): 2.9e-05, (40.0% identity in 65 aa overlap). Also similar to mce-associated proteins from Mycobacterium tuberculosis e.g. Rv1363c, Rv0177, Rv3493c, etc. Protein product from Mb0182 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0182 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTVVVEKTPT TLPQATPNGA APWHVRAGAF AIDVLPGLAV AATMALTALT VPPGSAWRWL 61 CACLLGLTIL LLAVNRLLLP TITGWSLGRA LTGIRVVRRD GSAIGPWRLL VRDLAHLVDT 121 LSLFVGWLWP LWDSRRRTFA DLLLRTEVRR VEPVQRPAVI RRLTAAVALA AAGACASATA 181 VGAAVVYVNE WQTDHTRAQL ATRGPKLVVD VLSYDPETVQ RDFERARSLA TDRYRPQLSI 241 QQDSVRESGP VRNQYWVTDS AVLSATPAQA TMLLFMQGER GTPPNQRYIS ATVRAIFQKS 301 RGQWRLDDLA VVMKPRQPTG EK //