LOCUS CAB5247885.1 515 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 MCE- FAMILY PROTEIN MCE1F protein. ACCESSION LT708304-184 PROTEIN_ID CAB5247885.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mce1F" /locus_tag="BQ2027_MB0180" /note="Mb0180, mce1F, len: 515 aa. Equivalent to Rv0174, len: 515 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 515 aa overlap). mce1F; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below for more information), similar to Mycobacterium tuberculosis proteins O07784|Rv0594|MTCY19H5.28c|mce2F (516 aa); O53972|Rv1971|MTV051.09|mce3F (437 aa); etc. Also highly similar to others e.g. NP_302661.1|NC_002677 putative secreted protein from Mycobacterium leprae (516 aa); AAF74993.1|AF143400_1|AF143400|996A027a protein from Mycobacterium avium (80 aa) (similarity on C-terminus); CAC12793.1|AL445327 putative secreted protein from Streptomyces coelicolor (433 aa); etc. Has hydrophobic stretch, possibly a signal peptide at the N-terminus. Protein product from Mb0180 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0180 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MLTRFIRRQL ILFAIVSVVA IVVLGWYYLR IPSLVGIGQY TLKADLPASG GLYPTANVTY 61 RGITIGKVTA VEPTDQGARV TMSIASNYKI PVDASANVHS VSAVGEQYID LVSTGAPGKY 121 FSSGQTITKG TVPSEIGPAL DNSNRGLAAL PTEKIGLLLD ETAQAVGGLG PALQRLVDST 181 QAIVGDFKTN IGDVNDIIEN SGPILDSQVN TGDQIERWAR KLNNLAAQTA TRDQNVRSIL 241 SQAAPTADEV NAVFSGVRDS LPQTLANLEV VFDMLKRYHA GVEQLLVFLP QGAAIAQTVL 301 TPTPGAAQLP LAPAINYPPP CLTGFLPASE WRSPADTSPR PLPSGTYCKI PQDAQLQVRG 361 ARNIPCVDVP GKRAATPKEC RSKDPYVPLG TNPWFGDPNQ ILTCPAPGAR CDQPVKPGLV 421 IPAPSINTGL NPAPADQVQG TPPPVSDPLQ RPGSGTVQCN GQQPNPCVYT PTSGPSAVYS 481 PASGELVGPD GVKYAVANSS TTGDDGWKEM LAPAS //