LOCUS CAB5247883.1 530 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 MCE- FAMILY PROTEIN MCE1D protein. ACCESSION LT708304-182 PROTEIN_ID CAB5247883.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mce1D" /locus_tag="BQ2027_MB0178" /note="Mb0178, mce1D, len: 530 aa. Equivalent to Rv0172, len: 530 aa, fromMycobacterium tuberculosis strain H37Rv, (100.0% identity in 530 aa overlap). mce1D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below for more information), highly similar to Mycobacterium tuberculosis proteins O07786|Rv0592|MTCY19H5.30c|mce2D (508 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Hydrophobic region at N-terminus. Protein product from Mb0178 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0178 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSTIFDIRNL RLPQLSRASV VIGSLVVVLA LAAGIVGVRL YQKLTNNTVV AYFTQANALY 61 VGDKVQIMGL PVGSIDKIEP AGDKMKVTFH YQNKYKVPAN ASAVILNPTL VASRNIQLEP 121 PYRGGPVLAD NAVIPVERTQ VPTEWDELRD SVSHIIDELG PTPEQPKGPF GEVIEAFADG 181 LAGKGKQINT TLNSLSQALN ALNEGRGDFF AVVRSLALFV NALHQDDQQF VALNKNLAEF 241 TDRLTHSDAD LSNAIQQFDS LLAVARPFFA KNREVLTHDV NNLATVTTTL LQPDPLDGLE 301 TVLHIFPTLA ANINQLYHPT HGGVVSLSAF TNFANPMEFI CSSIQAGSRL GYQESAELCA 361 QYLAPVLDAI KFNYFPFGLN VASTASTLPK EIAYSEPRLQ PPNGYKDTTV PGIWVPDTPL 421 SHRNTQPGWV VAPGMQGVQV GPITQGLLTP ESLAELMGGP DIAPPSSGLQ TPPGPPNAYD 481 EYPVLPPIGL QAPQVPIPPP PPGPDVIPGP VPPTPAPVGA PLPAEAGGGQ //