LOCUS CAB5247881.1 346 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 MCE- FAMILY PROTEIN MCE1B protein. ACCESSION LT708304-180 PROTEIN_ID CAB5247881.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mce1B" /locus_tag="BQ2027_MB0176" /standard_name="mceD" /note="Mb0176, mce1B, len: 346 aa. Equivalent to Rv0170, len: 346 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 346 aa overlap). mce1B (alternate gene name: mceD); belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below for more information), highly similar to Mycobacterium tuberculosis proteins O07788|Rv0590|MTCY19H5.32c|mce2B (275 aa); O53968|Rv1967|MTV051.05|mce3B (342 aa); etc. Also highly similar to others e.g. NP_302657.1|NC_002677 putative secreted protein from Mycobacterium leprae (346 aa); CAC12797.1|AL445327 putative secreted protein from Streptomyces coelicolor (354 aa); etc. Contains hydrophobic region in N-terminal 30 residues. In Escherichia coli, N-terminal part is functional and directs export of a leaderless beta-lactamase into the periplasm (see seventh citation). Protein product from Mb0176 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0176 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MKITGTVVKL GIVSVVLLFF TVMIIVIFGQ MRFDRTNGYT AEFSNVSGLR QGQFVRASGV 61 EIGKVKALHL VDGGRRVRVE FNIDRSVPLY QSTTAQIRYS DLIGNRYVEL KRGEGKGAND 121 LLPPGGLIPL SRTSPALDLD ALIGGFKPVF RALDPAKVNN IANALITVFQ GQGGTINDTL 181 DQTAQLTSQI AERDQAIGEV VKNLNIVLDT TVKHRKEFDE TVNNLENLIT GLRNHSDQLA 241 GGLAHISNGA GTVADLLAEN RTLVRKAVSY LDAIQQPVID QRVELDDLLH KTPTALTALG 301 RANGTYGDFQ NFYLCDLQIK WNGFQAGGPV RTVKLFSQPT GRCTPQ //