LOCUS CAB5247877.1 554 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE FATTY-ACID-CoA LIGASE FADD5 (FATTY-ACID-CoA SYNTHETASE) (FATTY-ACID-CoA SYNTHASE) protein. ACCESSION LT708304-176 PROTEIN_ID CAB5247877.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD5" /locus_tag="BQ2027_MB0172" /note="Mb0172, fadD5, len: 554 aa. Equivalent to Rv0166, len: 554 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 554 aa overlap). Probable fadD5, fatty-acid-CoA synthetase (EC 6.2.1.-), similar to many eg LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase (561 aa), FASTA scores: opt: 612, E(): 0, (29.4% identity in 534 aa overlap). Also similar to many other fatty-acid-CoA ligases from Mycobacterium tuberculosis e.g. MTCY07A7.11c FASTA score: (35.3% identity in 487 aa overlap), MTV013_10, MTY25D10_30, etc. Contains PS00455 putative AMP-binding domain signature. Protein product from Mb0172 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0172 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTAQLASHLT RALTLAQQQP YLARRQNWVN QLERHAMMQP DAPALRFVGN TMTWADLRRR 61 VAALAGALSG RGVGFGDRVM ILMLNRTEFV ESVLAANMIG AIAVPLNFRL TPTEIAVLVE 121 DCAAHVMLTE AALAPVAIGV RNIQPLLSVI VVAGGSSQDS VFGYEDLLNE AGDVHEPVDI 181 PNDSPALIMY TAGTTGRPKG AVLTHANLTG QAMTALYTSG ANINSDVGFV GVPLFHIAGI 241 GNMLTGLLLG LPTVIYPLGA FDPGQLLDVL EAEKVTGIFL VPAQWQAVCT EQQARPRDLR 301 LRVLSWGAAP APDALLRQMS ATFPETQILA AFGQTEMSPV TCMLLGEDAI AKRGSVGRVI 361 PTVAARVVDQ NMNDVPVGEV GEIVYRAPTL MSCYWNNPEA TAEAFAGGWF HSGDLVRMDS 421 DGYVWVVDRK KDMIISGGEN IYCAELENVL ASHPDIAEVA VIGRADEKWG EVPIAVAAVT 481 NDDLRIEDLG EFLTDRLARY KHPKALEIVD ALPRNPAGKV LKTELRLRYG ACVNVERRSA 541 SAGFTERREN RQKL //