LOCUS CAB5247873.1 383 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable zinc-type alcohol dehydrogenase (e subunit) adhe1 protein. ACCESSION LT708304-172 PROTEIN_ID CAB5247873.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="adhE1" /locus_tag="BQ2027_MB0167C" /note="Mb0167c, adhE1, len: 383 aa. Equivalent to Rv0162c, len: 383 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 383 aa overlap). Probable adhE1, zinc-type alcohol dehydrogenase (EC 1.1.1.1), similar to others e.g. ADH_MACMU|P28469 alcohol dehydrogenase alpha chain (374 aa), FASTA scores: opt: 619, E(): 0, (34.7% identity in 363 aa overlap). Also similar to other alcohol dehydrogenases from Mycobacterium tuberculosis e.g. MTCY369.06c FASTA score: (34.0% identity in 365 aa overlap), MTV022_9 FASTA score: (35.0% identity in 371 aa overlap). Contains PS00059 Zinc-contain ingalcohol dehydrogenases signature. BELONGS TO THE ZINC-CONTAINING ALCOHOL DEHYDROGENASE FAMILY, CLASS-I SUBFAMILY. COFACTOR: ZINC. Protein product from Mb0167c detected using SWATH mass spectrometry. Mb0167c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MPAVQPWLYS NMPAIRGAVL DQIGVPRPYW RSKPISVVEL HLDPPDRGEV LVRIEAAGVC 61 HSDLSVVDGT RVRPVPILLG HEAAGIVEQV GDGVDGVAVG QRVVLVFLPR CGQCAACATD 121 GRTPCEPGSA ANKAGTLLGG GIRLSRGGRP VYHHLGVSGF ATHVVVNRAS VVPVPHEVPP 181 TVAALLGCAV LTGGGAVLNV GDPQPGQSVA VVGLGGVGMA AVLTALTYTD VRVVAVDQLP 241 EKLSAAKALG AHEIYTPQQA TAGGVKAAVV VEAVGHPAAL HTAIGLTAPG GRTITVGLPP 301 PDVRISLSPL DFVTEGRSLI GSYLGSAVPS HDIPRFVSLW QSGRLPVESL VTSTIRLDDI 361 NEAMDHLADG IAVRQLISFT GDL //