LOCUS CAB5247858.1 286 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE protein. ACCESSION LT708304-157 PROTEIN_ID CAB5247858.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0153" /note="Mb0153, -, len: 286 aa. Equivalent to Rv0148, len: 286 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 286 aa overlap). Probable short-chain dehydrogenase (EC 1.-.-.-), similar to others, in particular Estradiol 17 beta-dehydrogenases (EC 1.1.1.62), e.g. DHB4_MOUSE|P51660 estradiol 17 beta-dehydrogenase 4 (735 aa), FASTA scores: opt: 952, E(): 0, (52.5% identity in 276 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature. BELONGS TO THE SHORT-CHAIN DEHYDROGENASES/REDUCTASES (SDR) FAMILY. Protein product from Mb0153 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0153 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MPGVQDRVIV VTGAGGGLGR EYALTLAGEG ASVVVNDLGG ARDGTGAGSA MADEVVAEIR 61 DKGGRAVANY DSVATEDGAA NIIKTALDEF GAVHGVVSNA GILRDGTFHK MSFENWDAVL 121 KVHLYGGYHV LRAAWPHFRE QSYGRVVVAT STSGLFGNFG QTNYGAAKLG LVGLINTLAL 181 EGAKYNIHAN ALAPIAATRM TQDILPPEVL EKLTPEFVAP VVAYLCTEEC ADNASVYVVG 241 GGKVQRVALF GNDGANFDKP PSVQDVAARW AEITDLSGAK IAGFKL //