LOCUS CAB5247857.1 506 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable aldehyde dehydrogenase (nad+) dependent protein. ACCESSION LT708304-156 PROTEIN_ID CAB5247857.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0152" /note="Mb0152, -, len: 506 aa. Equivalent to Rv0147, len: 506 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 506 aa overlap). Probable aldehyde dehydrogenase (NAD+) dependant (EC 1.2.1.-), similar to others e.g. DHAP_RAT|P11883 aldehyde dehydrogenase (dimeric NADP-preferring) (452 aa), FASTA scores: opt: 1291, E(): 0, (43.9% identity in 453 aa overlap). Also similar to several Mycobacterium tuberculosis aledehyde dehydrogenases e.g. Rv0768, Rv2858c, etc. Contains PS00687 aldehyde dehydrogenases glutamic acid active site, and PS00070 aldehyde dehydrogenases cysteine active site. BELONGS TO THE ALDEHYDE DEHYDROGENASES FAMILY. Protein product from Mb0152 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0152 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSDRVKAVAP PDGRTMMTTE SVARKTQKSE TEAPREPAPV SDEKQTDVAK TVARLRKTFA 61 SGRTRSVEWR KQQLRALQKL MDENEDAIAA ALAEDLDRNP FEAYLADIAT TSAEAKYAAK 121 RVRRWMRRRY LLLEVPQLPG RGWVEYEPYG TVLIIGAWNY PFYLTLGPAV GAIAAGNAVV 181 LKPSEIAAAS AHLMTELVYR YLDTEAIAVV QGDGAVSQEL IAQGFDRVMF TGGTEIGRKV 241 YEGAAPHLTP VTLELGGKSP VIVAADADVD VAAKRIAWIK LLNAGQTCVA PDYVLADATV 301 RDELVSKITA ALTKFRSGAP QGMRIVNQRQ FDRLSGYLAA AKTDAAADGG GVVVGGDCDA 361 SNLRIQPTVV VDPDPDGPLM SNEIFGPILP VVTVKSLDDA IRFVNSRPKP LSAYLFTKSR 421 AVRERVIREV PAGGMMVNHL AFQVSTAKLP FGGVGASGMG AYHGRWGFEE FSHRKSVLTK 481 PTRPDLSSFI YPPYTERAIK VARRLF //