LOCUS CAB5247853.1 492 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED TRANSMEMBRANE PROTEIN protein. ACCESSION LT708304-152 PROTEIN_ID CAB5247853.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0148C" /note="Mb0148c, -, len: 492 aa. Equivalent to Rv0143c, len: 492 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 492 aa overlap). Probable conserved transmembrane protein, CIC family possibly involved in transport of chloride, similar to others and hypothetical proteins e.g. O28857 PUTATIVE CHLORIDE CHANNEL from Archaeoglobus fulgidus (589 aa), FASTA scores: opt: 966, E(): 0, (37.7% identity in 453 aa overlap); YADQ_ECOLI|P37019 hypothetical 46.0 kd protein (436 aa), FASTA scores: opt: 452, E(): 2.4e-20, (28.0% identity in 460 aa overlap). Protein product from Mb0148c detected using SWATH mass spectrometry. Mb0148c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MAPGDWSVFA WHAANLPTMP EAEDIGNEAA GGRLGVSIRS AGYLRKWFLL GITIGVIAGL 61 GAVVFYLALK YTSEFLLGYL ADYQIPTPVG EGGHRGSTGF ARPWAIPLVT TGGAVLSALI 121 VAKLAPEATG HGTDEAIESV HGDPRAIRGR AVLVKMVASA LTIGSGGSGG REGPTAQISA 181 GFCSLLTRRL NLSNEDGRTA VALGIGAGIG AIFAAPLGGA ALGASIPYRD DFDYRNLLPG 241 FIASGTAYAV LGAFLGFDPL FGYIDAEYRF EKAWPLLWFV VIGLIAAAVG YLYARVFHAS 301 VAITRRLPGG PVLKPAIGGL LVGLLGLPIP QILSSGYGWA QLAADRGTLL SIPLWIVIVL 361 PIAKILATSL SIGTGGSGGL FGPGIVIGAF VGAAIWRLGE LTELPGVPHE PGIFVVVAMM 421 ACFGSVSRAP LAVMIMVAEM TGSFSVVPGA IIAVGIAALL LSRTNVTIYE TQRLNRQTAE 481 AERGGSDRPT TA //