LOCUS CAB5247834.1 455 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 maltokinase mak protein. ACCESSION LT708304-133 PROTEIN_ID CAB5247834.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mak" /locus_tag="BQ2027_MB0132" /note="Mb0132, -, len: 455 aa. Equivalent to Rv0127, len: 455 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 455 aa overlap). Conserved hypothetical protein, highly similar to various proteins e.g. AJ0012|SCJ001205_4 hypothetical protein from Streptomyces coelicolor A3(2) (464 aa), FASTA scores: opt: 412, E(): 1.1e-19, (40.6% identity in 485 aa overlap); AJ0012|SCJ001206_5 hypothetical protein from Streptomyces coelicolor A3(2) (453 aa), FASTA scores: opt: 403, E(): 4.3 e-19, (36.5% identity in 455 aa overlap). Protein product from Mb0132 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0132 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MTRSDTLATK LPWSDWLPRQ RWYAGRNREL ATVKPGVVVA LRHNLDLVLV DVTYTDGATE 61 RYQVLVGWDF EPASEYGTKA AIGVADDRTG FDALYDVAGP QFLLSLIVSS AVCGTSTGEV 121 TFTREPDVEL PFAAQPRVCD AEQSNTSVIF DRRAILKVFR RVSSGINPDI ELNRVLTRAG 181 NPHVARLLGA YQFGRPNRSP TDALAYALGM VTEYEANAAE GWAMATASVR DLFAEGDLYA 241 HEVGGDFAGE SYRLGEAVAS VHATLADSLG TAQATFPVDR MLARLSSTVA VVPELREYAP 301 TIEQQFQKLA AEAITVQRVH GDLHLGQVLR TPESWLLIDF EGEPGQPLDE RRAPDSPLRD 361 VAGVLRSFEY AAYGPLVDQA TDKQLAARAR EWVERNRAAF CDGYAVASGI DPRDSALLLG 421 AYELDKAVYE TGYETRHRPG WLPIPLRSIA RLTAS //