LOCUS CAB5247833.1 601 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 TREHALOSE SYNTHASE TRES protein. ACCESSION LT708304-132 PROTEIN_ID CAB5247833.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="treS" /locus_tag="BQ2027_MB0131" /note="Mb0131, treS, len: 601 aa. Equivalent to Rv0126, len: 601 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 601 aa overlap). treS, trehalose synthase (EC 5.4.99.-) (see citation below), highly similar to others e.g. CAA04601.2|AJ001205 putative trehalose synthase from Streptomyces coelicolor (566 aa); S71450|1536814|BAA11303.1|D78198 trehalose synthase maltose-specific from Pimelobacter sp. strain R48 (573 aa). Also similar to MAL1_DROME|P07191 possible maltase precursor (508 aa), FASTA scores: opt: 807, E(): 0, (33.7% identity in 504 aa overlap); and similar to proteins associated with amino-acid transport e.g. Q64319 rat protein which stimulates transport of cystine and dibasic and neutral amino acids (683 aa), FASTA scores: opt: 839, E(): 0, (32.0% identity in 531 aa overlap). Also similar to several other Mycobacterium tuberculosis proteins e.g. Rv2471 FASTA score: (31.7% identity in 164 aa overlap). Protein product from Mb0131 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0131 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNEAEHSVEH PPVQGSHVEG GVVEHPDAKD FGSAAALPAD PTWFKHAVFY EVLVRAFFDA 61 SADGSGDLRG LIDRLDYLQW LGIDCIWLPP FYDSPLRDGG YDIRDFYKVL PEFGTVDDFV 121 ALVDAAHRRG IRIITDLVMN HTSESHPWFQ ESRRDPDGPY GDYYVWSDTS ERYTDARIIF 181 VDTEESNWSF DPVRRQFYWH RFFSHQPDLN YDNPAVQEAM IDVIRFWLGL GIDGFRLDAV 241 PYLFEREGTN CENLPETHAF LKRVRKVVDD EFPGRVLLAE ANQWPGDVVE YFGDPNTGGD 301 ECHMAFHFPL MPRIFMAVRR ESRFPISEII AQTPPIPDMA QWGIFLRNHD ELTLEMVTDE 361 ERDYMYAEYA KDPRMKANVG IRRRLAPLLD NDRNQIELFT ALLLSLPGSP VLYYGDEIGM 421 GDVIWLGDRD GVRIPMQWTP DRNAGFSTAN PGRLYLPPSQ DPVYGYQAVN VEAQRDTSTS 481 LLNFTRTMLA VRRRHPAFAV GAFQELGGSN PSVLAYVRQV AGDDGDTVLC VNNLSRFPQP 541 IELDLQQWTN YTPVELTGHV EFPRIGQVPY LLTLPGHGFY WFQLTTHEVG APPTCGGERR 601 L //