LOCUS       CAB5247832.1             355 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 probable
            serine protease pepa (serine proteinase) (mtb32a) protein.
ACCESSION   LT708304-131
PROTEIN_ID  CAB5247832.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="pepA"
                     /locus_tag="BQ2027_MB0130"
                     /note="Mb0130, pepA, len: 355 aa. Equivalent to Rv0125,
                     len: 355 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 355 aa overlap). Probable pepA, serine
                     protease (EC 3.4.21.-), highly similar to other proteases
                     e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa),
                     FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in
                     346 aa overlap). Also similar to Q50320 34 KDA protein
                     precursor from Mycobacterium tuberculosis (361 aa), FASTA
                     scores: opt: 1689, E(): 0, (70.7% identity in 362 aa
                     overlap). Contains PS00135 Serine proteases, trypsin
                     family, serine active site. Has a possible signal sequence
                     at the N-terminus. Protein product from Mb0130 detected
                     using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb0130 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MSNSRRRSLR WSWLLSVLAA VGLGLATAPA QAAPPALSQD RFADFPALPL DPSAMVAQVG
       61 PQVVNINTKL GYNNAVGAGT GIVIDPNGVV LTNNHVIAGA TDINAFSVGS GQTYGVDVVG
      121 YDRTQDVAVL QLRGAGGLPS AAIGGGVAVG EPVVAMGNSG GQGGTPRAVP GRVVALGQTV
      181 QASDSLTGAE ETLNGLIQFD AAIQPGDSGG PVVNGLGQVV GMNTAASDNF QLSQGGQGFA
      241 IPIGQAMAIA GQIRSGGGSP TVHIGPTAFL GLGVVDNNGN GARVQRVVGS APAASLGIST
      301 GDVITAVDGA PINSATAMAD ALNGHHPGDV ISVTWQTKSG GTRTGNVTLA EGPPA
//