LOCUS CAB5247832.1 355 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable serine protease pepa (serine proteinase) (mtb32a) protein. ACCESSION LT708304-131 PROTEIN_ID CAB5247832.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pepA" /locus_tag="BQ2027_MB0130" /note="Mb0130, pepA, len: 355 aa. Equivalent to Rv0125, len: 355 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 355 aa overlap). Probable pepA, serine protease (EC 3.4.21.-), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 KDA protein precursor from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a possible signal sequence at the N-terminus. Protein product from Mb0130 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0130 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSNSRRRSLR WSWLLSVLAA VGLGLATAPA QAAPPALSQD RFADFPALPL DPSAMVAQVG 61 PQVVNINTKL GYNNAVGAGT GIVIDPNGVV LTNNHVIAGA TDINAFSVGS GQTYGVDVVG 121 YDRTQDVAVL QLRGAGGLPS AAIGGGVAVG EPVVAMGNSG GQGGTPRAVP GRVVALGQTV 181 QASDSLTGAE ETLNGLIQFD AAIQPGDSGG PVVNGLGQVV GMNTAASDNF QLSQGGQGFA 241 IPIGQAMAIA GQIRSGGGSP TVHIGPTAFL GLGVVDNNGN GARVQRVVGS APAASLGIST 301 GDVITAVDGA PINSATAMAD ALNGHHPGDV ISVTWQTKSG GTRTGNVTLA EGPPA //