LOCUS       CAB5247827.1             117 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ELONGATION FACTOR G FUSA2A [FIRST PART] (EF-G) protein.
ACCESSION   LT708304-126
PROTEIN_ID  CAB5247827.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fusA2a"
                     /locus_tag="BQ2027_MB0125C"
                     /standard_name="fus2"
                     /note="Mb0125c, fusA2a, len: 117 aa. Equivalent to 5' end
                     of Rv0120c, len: 714 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (98.1% identity in 108 aa overlap). Probable
                     fusA2 (alternate gene name: fus2), elongation factor G,
                     highly similar to others e.g. EFG_ECOLI|P02996 elongation
                     factor G (ef-g) from Escherichia coli (703 aa), FASTA
                     scores: opt: 1049, E(): 0, (32.5% identity in 717 aa
                     overlap). Also similar to fusA1|MTCY210.01 from
                     Mycobacterium tuberculosis FASTA score: (39.1% identity in
                     299 aa overlap); and P30767|EFG_MYCLE ELONGATION FACTOR G
                     (EF-G) from Mycobacterium leprae (701 aa), FASTA score:
                     (31.7% identity in 710 aa overlap). Contains PS00017
                     ATP/GTP-binding site motif A (P-loop). BELONGS TO THE
                     GTP-BINDING ELONGATION FACTOR FAMILY, EF-G/EF-2 SUBFAMILY.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, fusA2 exists as a single gene.
                     In Mycobacterium bovis, a frameshift due to the
                     substitution of a single base (gc-t), splits fusA2 into 2
                     parts, fusA2a and fusA2b. Protein product from Mb0125c
                     detected using shotgun mass spectrometry. Mb0125c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MADRVNASQG AAAAPTANGP GGVRNVVLVG PSGGGKTTLI EALLVAAKVL SRPGSVTEGT
       61 TVCDFDEAEI RQQRSVGLAV ASLAYDGIKV NLVDTPGYAD FVGELWPGCG PPIAHCS
//