LOCUS CAB5247826.1 597 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ELONGATION FACTOR G FUSA2B [SECOND PART] (EF-G) protein. ACCESSION LT708304-125 PROTEIN_ID CAB5247826.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fusA2b" /locus_tag="BQ2027_MB0124C" /standard_name="fus2" /note="Mb0124c, fusA2b, len: 597 aa. Equivalent to 3' end of Rv0120c, len: 714 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 597 aa overlap). Probable fusA2 (alternate gene name: fus2), elongation factor G, highly similar to others e.g. EFG_ECOLI|P02996 elongation factor G (ef-g) from Escherichia coli (703 aa), FASTA scores: opt: 1049, E(): 0, (32.5% identity in 717 aa overlap). Also similar to fusA1|MTCY210.01 from Mycobacterium tuberculosis FASTA score: (39.1% identity in 299 aa overlap); and P30767|EFG_MYCLE ELONGATION FACTOR G (EF-G) from Mycobacterium leprae (701 aa), FASTA score: (31.7% identity in 710 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). BELONGS TO THE GTP-BINDING ELONGATION FACTOR FAMILY, EF-G/EF-2 SUBFAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, fusA2 exists as a single gene. In Mycobacterium bovis, a frameshift due to a 2bp to 1bp substitution (gc-t), splits fusA2 into 2 parts, fusA2a and fusA2b. Protein product from Mb0124c detected using SWATH mass spectrometry. Mb0124c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MIAANEGVDE PTKSLWQECS QVGMPRAVVI TKLDHARANY REALTAAQDA FGDKVLPLYL 61 PSGDGLIGLL SQALYEYADG KRTTRTPAES DTERIEEARG ALIEGIIEES EDESLMERYL 121 GGETIDESVL IQDLEKAVAR GSFFPVIPVC SSTGVGTLEL LEVATRGFPS PMEHPLPEVF 181 TPQGVPHAEL ACDNDAPLLA EVVKTTSDPY VGRVSLVRVF SGTIRPDTTV HVSGHFSSFF 241 GGGTSNTHPD HDEDERIGVL SFPLGKQQRP AAAVVAGDIC AIGKLSRAET GDTLSDKAEP 301 LVLKPWTMPE PLLPIAIAAH AKTDEDKLSV GLGRLAAEDP TLRIEQNQET HQVVLWCMGE 361 AHAGVVLDTL ANRYGVSVDT IELRVPLRET FAGNAKGHGR HIKQSGGHGQ YGVCDIEVEP 421 LPEGSGFEFL DKVVGGAVPR QFIPSVEKGV RAQMDKGVHA GYPVVDIRVT LLDGKAHSVD 481 SSDFAFQMAG ALALREAAAA TKVILLEPID EISVLVPDDF VGAVLGDLSS RRGRVLGTET 541 AGHDRTVIKA EVPQVELTRY AIDLRSLAHG AASFTRSFAR YEPMPESAAA RVKAGAG //