LOCUS       CAB5247826.1             597 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ELONGATION FACTOR G FUSA2B [SECOND PART] (EF-G) protein.
ACCESSION   LT708304-125
PROTEIN_ID  CAB5247826.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fusA2b"
                     /locus_tag="BQ2027_MB0124C"
                     /standard_name="fus2"
                     /note="Mb0124c, fusA2b, len: 597 aa. Equivalent to 3' end
                     of Rv0120c, len: 714 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (99.8% identity in 597 aa overlap). Probable
                     fusA2 (alternate gene name: fus2), elongation factor G,
                     highly similar to others e.g. EFG_ECOLI|P02996 elongation
                     factor G (ef-g) from Escherichia coli (703 aa), FASTA
                     scores: opt: 1049, E(): 0, (32.5% identity in 717 aa
                     overlap). Also similar to fusA1|MTCY210.01 from
                     Mycobacterium tuberculosis FASTA score: (39.1% identity in
                     299 aa overlap); and P30767|EFG_MYCLE ELONGATION FACTOR G
                     (EF-G) from Mycobacterium leprae (701 aa), FASTA score:
                     (31.7% identity in 710 aa overlap). Contains PS00017
                     ATP/GTP-binding site motif A (P-loop). BELONGS TO THE
                     GTP-BINDING ELONGATION FACTOR FAMILY, EF-G/EF-2 SUBFAMILY.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, fusA2 exists as a single gene.
                     In Mycobacterium bovis, a frameshift due to a 2bp to 1bp
                     substitution (gc-t), splits fusA2 into 2 parts, fusA2a and
                     fusA2b. Protein product from Mb0124c detected using SWATH
                     mass spectrometry. Mb0124c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MIAANEGVDE PTKSLWQECS QVGMPRAVVI TKLDHARANY REALTAAQDA FGDKVLPLYL
       61 PSGDGLIGLL SQALYEYADG KRTTRTPAES DTERIEEARG ALIEGIIEES EDESLMERYL
      121 GGETIDESVL IQDLEKAVAR GSFFPVIPVC SSTGVGTLEL LEVATRGFPS PMEHPLPEVF
      181 TPQGVPHAEL ACDNDAPLLA EVVKTTSDPY VGRVSLVRVF SGTIRPDTTV HVSGHFSSFF
      241 GGGTSNTHPD HDEDERIGVL SFPLGKQQRP AAAVVAGDIC AIGKLSRAET GDTLSDKAEP
      301 LVLKPWTMPE PLLPIAIAAH AKTDEDKLSV GLGRLAAEDP TLRIEQNQET HQVVLWCMGE
      361 AHAGVVLDTL ANRYGVSVDT IELRVPLRET FAGNAKGHGR HIKQSGGHGQ YGVCDIEVEP
      421 LPEGSGFEFL DKVVGGAVPR QFIPSVEKGV RAQMDKGVHA GYPVVDIRVT LLDGKAHSVD
      481 SSDFAFQMAG ALALREAAAA TKVILLEPID EISVLVPDDF VGAVLGDLSS RRGRVLGTET
      541 AGHDRTVIKA EVPQVELTRY AIDLRSLAHG AASFTRSFAR YEPMPESAAA RVKAGAG
//