LOCUS CAB5247818.1 318 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE GDP-MANNOSE 4,6-DEHYDRATASE GCA (GDP-D-MANNOSE DEHYDRATASE) protein. ACCESSION LT708304-117 PROTEIN_ID CAB5247818.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="gca" /locus_tag="BQ2027_MB0116" /note="Mb0116, gca, len: 318 aa. Equivalent to Rv0112, len: 318 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 318 aa overlap). Possible gca, GDP-mannose 4,6-dehydratase (EC 4.2.1.47), similar to others e g. U18320|PAU18320_1 GDP-D-mann from Pseudomonas aeruginosa (323 aa), FASTA scores: opt: 415, E(): 4.4e-21, (27.0% identity in 318 aa overlap). Similar to Rv3634c, Rv3784, etc from Mycobacterium tuberculosis. Contains PS00061 Short-chain dehydrogenases/reductases family signature. SEEMS TO BELONG TO THE GDP-MANNOSE 4,6-DEHYDRATASE FAMILY. COFACTOR: NAD(+). Protein product from Mb0116 detected using SWATH mass spectrometry. Mb0116 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MKVWITGAGG MMGSHLAEML LAAGHDVYAT YCRPTIDPSD LQFNGAEVDI TDWCSVYDSI 61 ATFRPDAVFH LAAQSYPAVS WARPVETLTT NMVGTAIVFE ALRRVRPHAK IIVAGSSAEY 121 GFVDPSEVPI NERRELRPLH PYGVSKAATD MLAYQYHKSY GMHTVVARIF NCTGPRKVGD 181 ALSDFVRRCT WLEHHPEQSA IRVGNLKTKR TIVDVRDLNR ALMLMLDKGE AGADYNVGGS 241 IAYEMGDVLK QVIAACKRDD IVPEVDPALL RPTDEKIIYG DCSKLAAITG WQQEICLTQT 301 IADMFDYWRS KSESALMV //