LOCUS CAB5247807.1 2512 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
PEPTIDE SYNTHETASE NRP (PEPTIDE SYNTHASE) protein.
ACCESSION LT708304-106
PROTEIN_ID CAB5247807.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="nrp"
/locus_tag="BQ2027_MB0104"
/note="Mb0104, nrp, len: 2512 aa. Equivalent to Rv0101,
len: 2512 aa, from Mycobacterium tuberculosis strain
H37Rv, (100.0% identity in 2512 aa overlap). Probable nrp,
peptide synthetase (EC 6.-.-.-), similar to others e.g.
AAD44234.1|AF143772_40|PstB peptide synthetase from
Mycobacterium avium (2552 aa); 7476034|S77657 cyclic
peptide synthetase from Mycobacterium leprae (1401 aa),
FASTA scores: opt: 4268, E(): 0, (65.7% identity in 1091
aa overlap); part of CAB55600.1|AJ238027 peptide
synthetase from Mycobacterium smegmatis (5990). Also
similar to e.g. AAD56240.1|AF184977_1|AF184977 DhbF
protein from Bacillus subtilis (2378 aa);
SRF1_BACSU|P27206 surfactin synthetase subunit 1 (3587
aa), FASTA scores: opt: 1708, E(): 0, (30.6% identity in
1633 aa overlap): etc. Contains PS00017 ATP/GTP-binding
site motif A (P-loop), 2 x PS00455 Putative AMP-binding
domain signature, and PS00012 Phosphopantetheine
attachment site. BELONGS TO THE ATP-DEPENDENT AMP-BINDING
ENZYME FAMILY. THOUGHT TO BE NOT INVOLVED IN MYCOBACTIN
BIOSYNTHESIS (see citation below). Protein product from
Mb0104 detected using shotgun mass spectrometry. Mb0104
found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing (TPM > 20)."
BEGIN
1 MHRVRLSRSQ RNLYNGVRQD NNPALYLIGK SYRFRRLELA RFLAALHATV LDNPVQLCVL
61 ENSGADYPDL VPRLRFGDIV RVGSADEHLQ STWCSGILGK PLVRHTVHTD PNGYVTGLDV
121 HTHHILLDGG ATGTIEADLA RYLTTDPAGE TPSVGAGLAK LREAHRRETA KVEESRGRLS
181 AVVQRELADE AYHGGHGHSV SDAPGTAAKG VLHESATICG NAFDAILTLS EAQRVPLNVL
241 VAAAAVAVDA SLRQNTETLL VHTVDNRFGD SDLNVATCLV NSVAQTVRFP PFASVSDVVR
301 TLDRGYVKAV RRRWLREEHY RRMYLAINRT SHVEALTLNF IREPCAPGLR PFLSEVPIAT
361 DIGPVEGMTV ASVLDEEQRT LNLAIWNRAD LPACKTHPKV AERIAAALES MAAMWDRPIA
421 MIVNDWFGIG PDGTRCQGDW PARQPSTPAW FLDSARGVHQ FLGRRRFVYP WVAWLVQRGA
481 APGDVLVFTD DDTDKTIDLL IACHLAGCGY SVCDTADEIS VRTNAITEHG DGILVTVVDV
541 AATQLAVVGH DELRKVVDER VTQVTHDALL ATKTAYIMPT SGTTGQPKLV RISHGSLAVF
601 CDAISRAYGW GAHDTVLQCA PLTSDISVEE IFGGAACGAR LVRSAAMKTG DLAALVDDLV
661 ARETTIVDLP TAVWQLLCAD GDAIDAIGRS RLRQIVIGGE AIRCSAVDKW LESAASQGIS
721 LLSSYGPTEA TVVATFLPIV CDQTTMDGAL LRLGRPILPN TVFLAFGEVV IVGDLVADGY
781 LGIDGDGFGT VTAADGSRRR AFATGDRVTV DAEGFPVFSG RKDAVVKISG KRVDIAEVTR
841 RIAEDPAVSD VAVELHSGSL GVWFKSQRTR EGEQDAAAAT RIRLVLVSLG VSSFFVVGVP
901 NIPRKPNGKI DSDNLPRLPQ WSAAGLNTAE TGQRAAGLSQ IWSRQLGRAI GPDSSLLGEG
961 IGSLDLIRIL PETRRYLGWR LSLLDLIGAD TAANLADYAP TPDAPTGEDR FRPLVAAQRP
1021 AAIPLSFAQR RLWFLDQLQR PAPVYNMAVA LRLRGYLDTE ALGAAVADVV GRHESLRTVF
1081 PAVDGVPRQL VIEARRADLG CDIVDATAWP ADRLQRAIEE AARHSFDLAT EIPLRTWLFR
1141 IADDEHVLVA VAHHIAADGW SVAPLTADLS AAYASRCAGR APDWAPLPVQ YVDYTLWQRE
1201 ILGDLDDSDS PIAAQLAYWE NALAGMPERL RLPTARPYPP VADQRGASLV VDWPASVQQQ
1261 VRRIARQHNA TSFMVVAAGL AVLLSKLSGS PDVAVGFPIA GRSDPALDNL VGFFVNTLVL
1321 RVNLAGDPSF AELLGQVRAR SLAAYENQDV PFEVLVDRLK PTRALTHHPL IQVMLAWQDN
1381 PVGQLNLGDL QATPMPIDTR TARMDLVFSL AERFSEGSEP AGIGGAVEYR TDVFEAQAID
1441 VLIERLRKVL VAVAAAPERT VSSIDALDGT ERARLDEWGN RAVLTAPAPT PVSIPQMLAA
1501 QVARIPEAEA VCCGDASMTY RELDEASNRL AHRLAGCGAG PGECVALLFE RCAPAVVAMV
1561 AVLKTGAAYL PIDPANPPPR VAFMLGDAVP VAAVTTAGLR SRLAGHDLPI IDVVDALAAY
1621 PGTPPPMPAA VNLAYILYTS GTTGEPKGVG ITHRNVTRLF ASLPARLSAA QVWSQCHSYG
1681 FDASAWEIWG ALLGGGRLVI VPESVAASPN DFHGLLVAEH VSVLTQTPAA VAMLPTQGLE
1741 SVALVVAGEA CPAALVDRWA PGRVMLNAYG PTETTICAAI SAPLRPGSGM PPIGVPVSGA
1801 ALFVLDSWLR PVPAGVAGEL YIAGAGVGVG YWRRAGLTAS RFVACPFGGS GARMYRTGDL
1861 VCWRADGQLE FLGRTDDQVK IRGYRIELGE VATALAELAG VGQAVVIARE DRPGDKRLVG
1921 YATEIAPGAV DPAGLRAQLA QRLPGYLVPA AVVVIDALPL TVNGKLDHRA LPAPEYGDTN
1981 GYRAPAGPVE KTVAGIFARV LGLERVGVDD SFFELGGDSL AAMRVIAAIN TTLNADLPVR
2041 ALLHASSTRG LSQLLGRDAR PTSDPRLVSV HGDNPTEVHA SDLTLDRFID ADTLATAVNL
2101 PGPSPELRTV LLTGATGFLG RYLVLELLRR LDVDGRLICL VRAESDEDAR RRLEKTFDSG
2161 DPELLRHFKE LAADRLEVVA GDKSEPDLGL DQPMWRRLAE TVDLIVDSAA MVNAFPYHEL
2221 FGPNVAGTAE LIRIALTTKL KPFTYVSTAD VGAAIEPSAF TEDADIRVIS PTRTVDGGWA
2281 GGYGTSKWAG EVLLREANDL CALPVAVFRC GMILADTSYA GQLNMSDWVT RMVLSLMATG
2341 IAPRSFYEPD SEGNRQRAHF DGLPVTFVAE AIAVLGARVA GSSLAGFATY HVMNPHDDGI
2401 GLDEYVDWLI EAGYPIRRID DFAEWLQRFE ASLGALPDRQ RRHSVLPMLL ASNSQRLQPL
2461 KPTRGCSAPT DRFRAAVRAA KVGSDKDNPD IPHVSAPTII NYVTNLQLLG LL
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