LOCUS CAB5247805.1 540 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE FATTY-ACID-COA LIGASE FADD10 (FATTY-ACID-COA SYNTHETASE) (FATTY-ACID-COA SYNTHASE) protein. ACCESSION LT708304-104 PROTEIN_ID CAB5247805.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD10" /locus_tag="BQ2027_MB0102" /note="Mb0102, fadD10, len: 540 aa. Equivalent to Rv0099, len: 540 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 540 aa overlap). Possible fadD10, fatty-acid-CoA synthetase (EC 6.2.1.-), equivalent to MLACEA_4|Q50176 LONG CHAIN FATTY ACID-COA LIGASE from Mycobacterium leprae (532 aa), FASTA scores: opt: 2580, E(): 0, (74.6% identity in 531 aa overlap). Also similar to many e.g. BAB69379.1|AB070955 long-chain fatty acid--CoA ligase from Streptomyces avermitilis (518 aa); NP_419782.1|NC_002696 putativ long-chain-fatty-acid--CoA ligase from Caulobacter crescentus (530 aa); NP_435326.1|NC_003037 probable long chain fatty acid CoA ligase from Sinorhizobium meliloti (508 aa); etc. Also similar to ACSA_BACSU|P39062 acetyl-coenzyme A synthetase from Bacillus subtilis (572 aa), FASTA scores: opt: 415, E(): 9.8e-20, (27.1% identity in 539 aa overlap). Contains PS00455 putative AMP-binding domain signature. BELONGS TO THE ATP-DEPENDENT AMP-BINDING ENZYME FAMILY. Protein product from Mb0102 detected using SWATH mass spectrometry. Mb0102 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MGGKKFQAMP QLPSTVLDRV FEQARQQPEA IALRRCDGTS ALRYRELVAE VGGLAADLRA 61 QSVSRGSRVL VISDNGPETY LSVLACAKLG AIAVMADGNL PIAAIERFCQ ITDPAAALVA 121 PGSKMASSAV PEALHSIPVI AVDIAAVTRE SEHSLDAASL AGNADQGSED PLAMIFTSGT 181 TGEPKAVLLA NRTFFAVPDI LQKEGLNWVT WVVGETTYSP LPATHIGGLW WILTCLMHGG 241 LCVTGGENTT SLLEILTTNA VATTCLVPTL LSKLVSELKS ANATVPSLRL VGYGGSRAIA 301 ADVRFIEATG VRTAQVYGLS ETGCTALCLP TDDGSIVKIE AGAVGRPYPG VDVYLAATDG 361 IGPTAPGAGP SASFGTLWIK SPANMLGYWN NPERTAEVLI DGWVNTGDLL ERREDGFFYI 421 KGRSSEMIIC GGVNIAPDEV DRIAEGVSGV REAACYEIPD EEFGALVGLA VVASAELDES 481 AARALKHTIA ARFRRESEPM ARPSTIVIVT DIPRTQSGKV MRASLAAAAT ADKARVVVRG //