LOCUS CAB5247797.1 255 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable bifunctional mta/sah nucleosidase mtn: 5'-methylthioadenosine nucleosidase (methylthioadenosine methylthioribohydrolase) + s-adenosylhomocysteine nucleosidase (s-adenosyl-l-homocysteine homocysteinylribohydrolase) protein. ACCESSION LT708304-96 PROTEIN_ID CAB5247797.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mtn" /locus_tag="BQ2027_MB0094" /note="Mb0094, mtn, len: 255 aa. Equivalent to Rv0091, len: 255 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 255 aa overlap). Probable mtn (alternate gene name: pfs), MTA/SAH nucleosidase, including 5'-methylthioadenosine nucleosidase (EC 3.2.2.16) and S-adenosylhomocysteine nucleosidase (EC 3.2.2.9), similar to others e.g. NP_521493.1|NC_003295 PROBABLE BIFUNCTIONAL PROTEIN (MTA/SAH NUCLEOSIDASE) (P46): 5'-METHYLTHIOADENOSINE NUCLEOSIDASE AND S-ADENOSYLHOMOCYSTEINE NUCLEOSIDASE from Ralstonia solanacearum (261 aa); AAC45731.1|U55214 Pfs from Treponema pallidum (249 aa); P96122|MTN_TREPA MTA/SAH NUCLEOSIDASE from Treponema pallidum (269 aa); PFS_ECOLI|P24247 pfs protein (p46) from Escherichia coli (232 aa), FASTA scores: opt: 214, E(): 3.8e-08, (30.5% identity in 246 aa overlap); etc. BELONGS TO THE MTN FAMILY. Protein product from Mb0094 detected using SWATH mass spectrometry. Mb0094 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MAVTVGVICA IPQELAYLRG VLVDAKRQQV AQILFDSGQL DAHRVVLAAA GMGKVNTGLT 61 ATLLADRFGC RTIVFTGVAG GLDPELCIGD IVIADRVVQH DFGLLTDERL RPYQPGHIPF 121 IEPTERLGYP VDPAVIDRVK HRLDGFTLAP LSTAAGGGGR QPRIYYGTIL TGDQYLHCER 181 TRNRLHHELG GMAVEMEGGA VAQICASFDI PWLVIRALSD LAGADSGVDF NRFVGEVAAS 241 SARVLLRLLP VLTAC //