LOCUS       CAB5247790.1             316 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            FORMATE HYDROGENLYASE HYCD (FHL) protein.
ACCESSION   LT708304-89
PROTEIN_ID  CAB5247790.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="hycD"
                     /locus_tag="BQ2027_MB0087"
                     /note="Mb0087, hycD, len: 316 aa. Equivalent to Rv0084,
                     len: 316 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 316 aa overlap). Possible hycD
                     (alternate gene name: hevD), formate hydrogenlyase (EC
                     1.-.-.-), integral membrane protein, similar to others
                     e.g. HYCD_ECOLI|P16430 formate hydrogenlyase subunit 4
                     from Escherichia coli (307 aa), FASTA scores: opt: 570,
                     E(): 2.1e-26, (33.8% identity in 305 aa overlap);
                     AAK06856.1|AF335723_2|AF335723 formate hydrogenlyase
                     subunit 4 from Burkholderia pseudomallei (316 aa);
                     NP_457244.1|NC_003198 formate hydrogenlyase subunit 4 from
                     Salmonella enterica subsp. enterica serovar Typhi (307
                     aa); etc. Also similar to NUOH_ECOLI|P33603 NADH
                     dehydrogenase I chain H from Escherichia coli (325 aa),
                     FASTA scores: opt: 207, E(): 9.5e-06, (26.5% identity in
                     260 aa overlap). BELONGS TO THE COMPLEX I SUBUNIT 1
                     FAMILY. Mb0087 found to be expressed during exponential
                     growth in Sauton's minimal media by RNA-sequencing (TPM >
                     20)."
BEGIN
        1 MSYLAGAAQI GGVMVGAPLV IGMTRQVRAR WEGRAGAGLL QPWRDLLKQL GKQQITPAGT
       61 TIVFAAAPVI VAGTTLLIAA IAPLVATGSP LDPSADLFAV VGLLFLGTVA LTLAGIDTGT
      121 SFGGMGASRE ITIAALVEPT ILLAVFALSI PAGSANLGAL VASTIDHPGH VVSLAGVLAF
      181 VALVIVIVAE TGRLPVDNPA THLELTMVHE AMVLEYAGPR LALVEWAAGM RLTVLLALLA
      241 NLFLPWGIAG AAPTALDVLT GVVAVAAKVA ILAVLLATFE VFLAKLRLFR VPELLAGSFL
      301 LALLAVTAAN FFTVGA
//