LOCUS CAB5247790.1 316 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE FORMATE HYDROGENLYASE HYCD (FHL) protein. ACCESSION LT708304-89 PROTEIN_ID CAB5247790.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="hycD" /locus_tag="BQ2027_MB0087" /note="Mb0087, hycD, len: 316 aa. Equivalent to Rv0084, len: 316 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 316 aa overlap). Possible hycD (alternate gene name: hevD), formate hydrogenlyase (EC 1.-.-.-), integral membrane protein, similar to others e.g. HYCD_ECOLI|P16430 formate hydrogenlyase subunit 4 from Escherichia coli (307 aa), FASTA scores: opt: 570, E(): 2.1e-26, (33.8% identity in 305 aa overlap); AAK06856.1|AF335723_2|AF335723 formate hydrogenlyase subunit 4 from Burkholderia pseudomallei (316 aa); NP_457244.1|NC_003198 formate hydrogenlyase subunit 4 from Salmonella enterica subsp. enterica serovar Typhi (307 aa); etc. Also similar to NUOH_ECOLI|P33603 NADH dehydrogenase I chain H from Escherichia coli (325 aa), FASTA scores: opt: 207, E(): 9.5e-06, (26.5% identity in 260 aa overlap). BELONGS TO THE COMPLEX I SUBUNIT 1 FAMILY. Mb0087 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MSYLAGAAQI GGVMVGAPLV IGMTRQVRAR WEGRAGAGLL QPWRDLLKQL GKQQITPAGT 61 TIVFAAAPVI VAGTTLLIAA IAPLVATGSP LDPSADLFAV VGLLFLGTVA LTLAGIDTGT 121 SFGGMGASRE ITIAALVEPT ILLAVFALSI PAGSANLGAL VASTIDHPGH VVSLAGVLAF 181 VALVIVIVAE TGRLPVDNPA THLELTMVHE AMVLEYAGPR LALVEWAAGM RLTVLLALLA 241 NLFLPWGIAG AAPTALDVLT GVVAVAAKVA ILAVLLATFE VFLAKLRLFR VPELLAGSFL 301 LALLAVTAAN FFTVGA //