LOCUS CAB5247776.1 280 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE GLUTAMINE-TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER GLNQ protein. ACCESSION LT708304-75 PROTEIN_ID CAB5247776.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0074" /note="Mb0074, -, len: 280 aa. Equivalent to 5' end of Rv0073, len: 330 aa, from Mycobacterium tuberculosis strain H37Rv, (94.9% identity in 275 aa overlap). Probable glutamine-transport ATP-binding protein ABC-transporter (see citation below), similar to many ATP-binding proteins e.g. NP_070646.1|NC_000917 ABC transporter ATP-binding protein from Archaeoglobus fulgidus (231 aa); T34822 ABC-transporter ATP binding protein from Streptomyces coelicolor (230 aa); YBJZ_ECOLI|P75831 hypothetical ABC transporter ATP-binding protein from Escherichia coli (648 aa), FASTA scores: opt: 531, E(): 6.8e-30, (38.6% identity in 233 aa overlap); etc. Also highly similar to Y0A4_MYCT|Q50734|MTCY9C4.04c hypothetical ABC transporter ATP-binding protein from Mycobacterium tuberculosis (330 aa), FASTA scores: E(): 0, (83.3% identity in 330 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature, and PS00889 Cyclic nucleotide-binding domain signature 2. BELONGS TO THE ATP-BINDING TRANSPORT PROTEIN FAMILY (ABC TRANSPORTERS). Note that supposed act with near ORF Rv0072|MTV030.16 transmembrane ABC-transporter. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv0073 exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base deletion (a-*) splits Rv0073 into 2 parts, Mb0074 and Mb0075. Mb0074 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MGDLSIQNLV VEYYSGGYAL RPINGLNLDV AAGSLVMLLG PSGCGKTTLL SCLGGILRPK 61 SGAIKFDEVD ITTLQGAELA NYRRNKVGIV FQAFNLVPSL TAVENVMVPL RSAGMSRRAS 121 RRRAEELLAR VNLAERMNHR PGDLSGGQQQ RVAVARAIAL DPPLILADEP TAHLDFIQVE 181 EVLRLIRELA DGERVVVVAT HDSRMLPMAD RVVELTPDFA ETNRPPETVH LQAGEVLFEQ 241 STMGDLIYVV SEGEFEIVHD WPTAVRNWSR LPGRGITSAR //