LOCUS       CAB40665.1                55 aa    PRT              VRL 18-APR-2005
DEFINITION  Human adenovirus 5 protein ( Adenovirus type 5 left 32%
            of the genome (coordinates 0% to 32.39% as measured by
            <ad2>). ).
ACCESSION   X02996-3
PROTEIN_ID  CAB40665.1
SOURCE      Human adenovirus 5
  ORGANISM  Human adenovirus 5
            Viruses; Adenoviridae; Mastadenovirus.
REFERENCE   1  (bases 1 to 194)
  AUTHORS   Steenbergh P.H., Maat J., Van ormondt H., Sussenbach J.S.
  TITLE     The nucleotide sequence at the termini of adenovirus type 5 DNA
  JOURNAL   Nucleic Acids Res. 4(12), 4371-4389(1977).
   PUBMED   600799
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Van ormondt H., Maat J., De Waard A., van der Eb A.J.
  TITLE     The nucleotide sequence of the transforming HpaI-E fragment of
            adenovirus type 5 DNA.
  JOURNAL   Gene 4(4), 309-328(1978).
   PUBMED   744489
REFERENCE   3  (bases 1575 to 2809)
  AUTHORS   Maat J., Van ormondt H.
  TITLE     The nucleotide sequence of the transforming hindiii-g fragment of
            adenovirus type 5 dna
  JOURNAL   Gene 6(1), 75-90(1979).
   PUBMED   478299
REFERENCE   4
  AUTHORS   Perricaudet M., Akusjarvi G., Virtanen A., Pettersson U.
  TITLE     Structure of two spliced mRNAs from the transforming region of
            human subgroup C adenoviruses
  JOURNAL   Nature 281(5733), 694-696(1979).
   PUBMED   551290
REFERENCE   5  (bases 10524 to 10696)
  AUTHORS   Thimmappaya B., Jones N., Shenk T.
  TITLE     A mutation which alters initiation of transcription by RNA
            polymerase III on the Ad5 chromosome
  JOURNAL   Cell 18(4), 947-954(1979).
   PUBMED   519773
REFERENCE   6  (bases 10555 to 10733)
  AUTHORS   Fowlkes D.M., Shenk T.
  TITLE     Transcriptional control regions of the adenovirus VAI RNA gene
  JOURNAL   Cell 22(2 Pt 2), 405-413(1980).
   PUBMED   7448868
REFERENCE   7  (bases 2804 to 4125)
  AUTHORS   Maat J., Van Beveren C.P., Van ormondt H.
  TITLE     The nucleotide sequence of adenovirus type 5 early region E1: the
            region between map positions 8.0 (HindIII site) and 11.8 (SmaI
            site)
  JOURNAL   Gene 10(1), 27-38(1980).
   PUBMED   6250944
REFERENCE   8  (bases 1 to 4125)
  AUTHORS   Van ormondt H., Maat J., Van Beveren C.P.
  TITLE     The nucleotide sequence of the transforming early region E1 of
            adenovirus type 5 DNA.
  JOURNAL   Gene 11(3-4), 299-309(1980).
   PUBMED   6260576
REFERENCE   9
  AUTHORS   Perricaudet M., Le Moullec J.M., Pettersson U.
  TITLE     Predicted structure of two adenovirus tumor antigens
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 77(7), 3778-3782(1980).
   PUBMED   6253988
REFERENCE   10  (bases 1 to 6246)
  AUTHORS   Broker T.R.
  TITLE     Appendix d: nucleotide sequences, transcription and translation
            analyses, and restriction endonuclease cleavage maps of group-c
            human adenoviruses
  JOURNAL   (in) Tooze J. (Eds.); DNA TUMOR VIRUSES:937-1002; Cold Spring
            Harbor Laboratory (1980)
REFERENCE   11  (bases 1653 to 4043)
  AUTHORS   Bos J.L., Polder L.J., Bernards R., Schrier P.I.,
            Van den elsen P.J., van der Eb A.J., Van ormondt H.
  TITLE     The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor
            antigens starting at different AUG triplets
  JOURNAL   Cell 27(1 Pt 2), 121-131(1981).
   PUBMED   7326748
REFERENCE   12  (bases 4001 to 6246)
  AUTHORS   Van Beveren C.P., Maat J., Dekker B.M., Van ormondt H.
  TITLE     The nucleotide sequence of the gene for protein IVa2 and of the 5'
            leader segment of the major late mRNAs of adenovirus type 5
  JOURNAL   Gene 16(1-3), 179-189(1981).
   PUBMED   7343420
REFERENCE   13  (bases 1 to 66)
  AUTHORS   Nagata K., Guggenheimer R.A., Hurwitz J.
  TITLE     Specific binding of a cellular DNA replication protein to the
            origin of replication of adenovirus DNA
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80(20), 6177-6181(1983).
   PUBMED   6336326
REFERENCE   14  (bases 325 to 604)
  AUTHORS   Hearing P., Shenk T.
  TITLE     Functional analysis of the nucleotide sequence surrounding the cap
            site for adenovirus type 5 region E1A messenger RNAs
  JOURNAL   J. Mol. Biol. 167(4), 809-822(1983).
   PUBMED   6876165
  REMARK    deletion mutants
REFERENCE   15
  AUTHORS   Downey J.F., Evelegh C.M., Branton P.E., Bayley S.T.
  TITLE     Peptide maps and N-terminal sequences of polypeptides from early
            region 1A of human adenovirus 5
  JOURNAL   J. Virol. 50(1), 30-37(1984).
   PUBMED   6699947
  REMARK    sites; cds start for E1a proteins
REFERENCE   16  (bases 6242 to 11570)
  AUTHORS   Dekker B.M.M., Van ormondt H.
  TITLE     The nucleotide sequence of fragment HindIII-C of human adenovirus
            type 5 DNA (map positions 17.1-31.7)
  JOURNAL   Gene 27(1), 115-120(1984).
   PUBMED   6325298
COMMENT     sites; splice sites in E1a 13S mRNA
            
            sites; splice sites and termini for E1b mRNAS
            
            Notes on the presentation of ADENO in the EMBL data library:
            
            The genetic map of Adeno is customarily presented from left to
            right, the 0% position being left and the 100% position being
            right. The two strands of Adeno are normally represented like this:
            
            r-strand:  3'------------------5'
            l-strand:  5'------------------3'
            ^                ^
            0%              100%
            
            This often causes confusion because the generally accepted way to
            represent DNA molecules is:
            
            5'------------------3'
            3'------------------5'
            
            Here Adeno virus sequences are always given in 5' to 3' direction
            and the sequence of the l-strand is displayed irrespective of the
            direction the viral transcription takes.
            
            This sequence corresponds to bases 1 to 11560 of <ad2>, which serve
            as some basis for the annotation of sites. the differences between
            <ad2> and <ad5> are too many to report herein, however a printout
            of those is available upon request from genbank. the map
            coordinates in the sites presume 360 bases per map unit. although
            there are approximately 115 sequence differences between the two
            strains over this region, no site difference exceeds 0.02% by this
            calculation.
            
            the sequence represents the early mrna transcripts e1a and e1b and
            the intermediate mrna transcript ix, all of which are transcribed
            rightwardly off the r-strand; the iva2 and e2b mrnas which are
            transcribed leftwardly off the l-strand ((indicated by "(c)" and
            "comp strand" below)); and the 5' end of the 28 kb major late mrna.
            the cap sites and possible promoter sequences for these are
            summarized in the following table:
            
            mrna        cap site           possible promoter region
            ------      ----------         --------------------------
            e1a           499              tatttata at bases 468-475 [6]
            e1b          1702              tatataa at bases 1672-1678[6]
            ix          3582              tatataa at bases 3551-3557 [6]
            iva2          5838 +/- 2  (c)   tacaaa at bases 5979-5974 on
            the comp strand [10]
            major         6049              tataaaa at bases 6018-6024 [10]
            
            as with ad2, not all the transcripts from this region have been
            characterized at the sequence level. the nine proteins given in
            features table below are not the only possible gene products (see
            the main adenovirus 2 entry).
            
            large amounts of small rnas are produced from the vai and vaii
            genes late in development for unknown reasons. [5] and [6]
            demonstrate that vai gene activity influences vaii expression; that
            the 5' flank affects the start site of the rna but that an
            intragenic promoter (bases 10626 to 10690 below) determines whether
            the rna is actually produced; and that there is striking similarity
            between this rna and trna.
FEATURES             Qualifiers
     source          /organism="Human adenovirus 5"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:28285"
     protein         /note="putative E1a protein from 9s mrna (splice sites
                     determined for ad2)"
                     /db_xref="GOA:P03255"
                     /db_xref="InterPro:IPR014410"
                     /db_xref="PDB:2KJE"
                     /db_xref="PDB:2R7G"
                     /db_xref="UniProtKB/Swiss-Prot:P03255"
     intron_pos      27:0 (1/1)
BEGIN
        1 MRHIICHGGV ITEEMAASLL DQLIEEVLCL NLSLSPSQNR SLQDLPAVLK WRLLS
//