LOCUS BAS37965.1 328 aa PRT BCT 15-SEP-2015 DEFINITION Klebsiella pneumoniae serine/threonine protein kinase protein. ACCESSION AP014950-5105 PROTEIN_ID BAS37965.1 SOURCE Klebsiella pneumoniae ORGANISM Klebsiella pneumoniae Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella. REFERENCE 1 (bases 1 to 5520319) AUTHORS Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and Mizunoe,Y. TITLE Direct Submission JOURNAL Submitted (14-SEP-2015) to the DDBJ/EMBL/GenBank databases. Contact:Tadayuki Iwase Jikei University School of Medicine, Bacteriology; 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan REFERENCE 2 AUTHORS Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and Mizunoe,Y. TITLE Complete genome sequence of Klebsiella pneumoniae YH43 JOURNAL Unpublished (2015) COMMENT The genome sequence of K. pneumoniae strain YH43 was determined using 454 GS FLX Titanium system. A total of 524,416 single-end reads (218 Mb) and 142,440 8-kb paired-end reads (46 Mb) were assembled with GS Assembler software version 2.6 into one scaffold containing 58 gaps. The finishing was first performed by in silico analysis using GenoFinisher software (http://www.ige.tohoku.ac.jp/joho/gf_e/) and then remaining gaps were closed by sequencing of gap-spanning PCR products using an ABI3130xl DNA sequencer. To correct sequence error by resequencing of YH43 genome, a paired-end library of YH43 was constructed using the TruSeq DNA Sample Prep kit and sequenced by the Illumina MiSeq platform (2x150 bp). The mapping of obtained 2,085,021 reads (626 Mb) to YH43 genome sequence and the SNPs-calling were performed using BWAand SAMtools, respectively. Total 38 in-dels were corrected by the resequencing. The gene identification and annotation were conducted by the Microbial Genome Annotation Pipeline (MiGAP). ##Genome-Assembly-Data-START## Assembly Method :: GS Assembler software v. 2.6 Genome Coverage :: 40x Sequencing Technolog :: 454 GS FLX; Illumina MiSeq ##Genome-Assembly-Data-END## FEATURES Qualifiers source /db_xref="taxon:573" /mol_type="genomic DNA" /organism="Klebsiella pneumoniae" /strain="YH43" protein /locus_tag="KPYH43_c5105" /transl_table=11 BEGIN 1 MHGKAFTFQT LRPDTIIDGL FDLGMRVDSG LTPLNSYENR VYQFQDEERH RYVVKFYRPE 61 RWSAEQILEE HLFALQLVED EVPVAAPLLF NDSTLHQHQG FYFAVFPSLG GRQFEADNLD 121 QMEWVGRYLG RLHQTGRKQR FTARPEIGVQ EYLLEPRQVF EQATLIPSGL KADFLKATDK 181 LIAAVMEQWH GCGNTLRLHG DCHAGNILWR DGPLFVDLDD ARTGPAIQDL WMLLNGDKAE 241 QRMQLETIVE AYEEFSPFNS DEIALIEPLR AMRLVYYLAW LLRRWDDPAF PVNFPWLTGE 301 DYWRGQTSTF LEQVKVLQEP PLQLTPMY //