LOCUS BAS36460.1 459 aa PRT BCT 15-SEP-2015 DEFINITION Klebsiella pneumoniae proline permease protein. ACCESSION AP014950-3600 PROTEIN_ID BAS36460.1 SOURCE Klebsiella pneumoniae ORGANISM Klebsiella pneumoniae Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella. REFERENCE 1 (bases 1 to 5520319) AUTHORS Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and Mizunoe,Y. TITLE Direct Submission JOURNAL Submitted (14-SEP-2015) to the DDBJ/EMBL/GenBank databases. Contact:Tadayuki Iwase Jikei University School of Medicine, Bacteriology; 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan REFERENCE 2 AUTHORS Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and Mizunoe,Y. TITLE Complete genome sequence of Klebsiella pneumoniae YH43 JOURNAL Unpublished (2015) COMMENT The genome sequence of K. pneumoniae strain YH43 was determined using 454 GS FLX Titanium system. A total of 524,416 single-end reads (218 Mb) and 142,440 8-kb paired-end reads (46 Mb) were assembled with GS Assembler software version 2.6 into one scaffold containing 58 gaps. The finishing was first performed by in silico analysis using GenoFinisher software (http://www.ige.tohoku.ac.jp/joho/gf_e/) and then remaining gaps were closed by sequencing of gap-spanning PCR products using an ABI3130xl DNA sequencer. To correct sequence error by resequencing of YH43 genome, a paired-end library of YH43 was constructed using the TruSeq DNA Sample Prep kit and sequenced by the Illumina MiSeq platform (2x150 bp). The mapping of obtained 2,085,021 reads (626 Mb) to YH43 genome sequence and the SNPs-calling were performed using BWAand SAMtools, respectively. Total 38 in-dels were corrected by the resequencing. The gene identification and annotation were conducted by the Microbial Genome Annotation Pipeline (MiGAP). ##Genome-Assembly-Data-START## Assembly Method :: GS Assembler software v. 2.6 Genome Coverage :: 40x Sequencing Technolog :: 454 GS FLX; Illumina MiSeq ##Genome-Assembly-Data-END## FEATURES Qualifiers source /db_xref="taxon:573" /mol_type="genomic DNA" /organism="Klebsiella pneumoniae" /strain="YH43" protein /locus_tag="KPYH43_c3600" /transl_table=11 BEGIN 1 MQQHKPHLLR GLNARHIRFI ALGSAIGTGL FYGSASAIKA AGPAVLLAYL IGGAAVFMVM 61 RALGEMAVRN PVSGSFGSYA RQYLGPLAGF ITGWTYTFEM VIVALADVTA FGIYMGLWYP 121 DVPRWIWILS IIFFIGAMNL CSVRVFGEME FWLSLIKVVA IIAMMAAGAG IIFFGFGHSF 181 PATGLENLWS HGGFAPNGWQ GIIASLGIVM FAFGGVEIIG VTAAEAQNPK KVIPQAINTI 241 PLRIILFYVC TLAVLMAIFP WNSFGEQGSP FVLIFDGLGI PAAATVLNII VISASISAIN 301 SDIFGAGRMM YGMSKEGLAP KCFQRIASNG VPWMTVVVMG VALLVAVVLN YLMPEQVFVL 361 IASLAAFATV WVWLMILLSH FSMRRGLSAE ERSKIEFPIP FWPVGPLLTL LFMGLVIAVL 421 GMVEETRVAL LAGLVWLGLL TVVWYARVRK TALQVATEQ //