LOCUS       BAS35179.1               501 aa    PRT              BCT 15-SEP-2015
DEFINITION  Klebsiella pneumoniae para-nitrobenzyl esterase protein.
ACCESSION   AP014950-2319
PROTEIN_ID  BAS35179.1
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5520319)
  AUTHORS   Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and
            Mizunoe,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2015) to the DDBJ/EMBL/GenBank databases.
            Contact:Tadayuki Iwase
            Jikei University School of Medicine, Bacteriology; 3-25-8
            Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan
REFERENCE   2
  AUTHORS   Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and
            Mizunoe,Y.
  TITLE     Complete genome sequence of Klebsiella pneumoniae YH43
  JOURNAL   Unpublished (2015)
COMMENT     The genome sequence of K. pneumoniae strain YH43 was determined
            using 454 GS FLX Titanium system. A total of 524,416 single-end
            reads (218 Mb) and 142,440 8-kb paired-end reads (46 Mb) were
            assembled with GS Assembler software version 2.6 into one scaffold
            containing 58 gaps. The finishing was first performed by in silico
            analysis using GenoFinisher software 
            (http://www.ige.tohoku.ac.jp/joho/gf_e/) and then remaining gaps
            were closed by sequencing of gap-spanning PCR products using an
            ABI3130xl DNA sequencer. To correct sequence error by resequencing
            of YH43 genome, a paired-end library of YH43 was constructed using
            the TruSeq DNA Sample Prep kit and sequenced by the Illumina MiSeq
            platform (2x150 bp). The mapping of obtained 2,085,021 reads (626
            Mb) to YH43 genome sequence and the SNPs-calling were performed
            using BWAand SAMtools, respectively. Total 38 in-dels were
            corrected by the resequencing. The gene identification and
            annotation were conducted by the Microbial Genome Annotation
            Pipeline (MiGAP).
            
            ##Genome-Assembly-Data-START##
            Assembly Method      :: GS Assembler software v. 2.6
            Genome Coverage      :: 40x
            Sequencing Technolog :: 454 GS FLX; Illumina MiSeq
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:573"
                     /mol_type="genomic DNA"
                     /organism="Klebsiella pneumoniae"
                     /strain="YH43"
     protein         /locus_tag="KPYH43_c2319"
                     /transl_table=11
BEGIN
        1 MQHPSKPLAK TRQGTVSGSA EQGIHIWRGI PYAAPPVGPL RWRAPQPPAR WQGVRQADAF
       61 SAASWQDIDY CRELGGGDPG AFSEDCLYLN IWAPAAAAQP LPVMVWLHGG GFTIGAGSLP
      121 PYDGKALASR GVVVVTVNYR LGHLGFFAHP ALEEEAGERL YNFALLDQIA ALQWVQDNIH
      181 AFGGDAANVT LFGESAGARS VLSLMASPKA KGLFHKAIIQ SGYTLPDLPR EKALEKGRLL
      241 AEHFALPQAS AEELRAIPAE AFWSLTAPLN TGPAPIVGDA VLPQPMLETF FAGRQHPMPV
      301 IIGSNSDEAS VMAVFGVDIA GQIQKLRRER RLGLGLIKLL YPGVKGDEAL GREVCRDMAF
      361 TTLGYVVMQA QQRVGQPCWR YWFDYVAEAE HDTYPHGAWH GNEVPYVFDN LRLTDPVCQY
      421 ASEADLAFAA QVADYWAQFA RVASGDQALA GAVRWPACLR GRDRLLRIGL HKRAGFKVEN
      481 RFMRARLALF RRVMKHHVTL E
//