LOCUS BAS33507.1 386 aa PRT BCT 15-SEP-2015 DEFINITION Klebsiella pneumoniae glutathionylspermidine synthase, group I protein. ACCESSION AP014950-647 PROTEIN_ID BAS33507.1 SOURCE Klebsiella pneumoniae ORGANISM Klebsiella pneumoniae Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella. REFERENCE 1 (bases 1 to 5520319) AUTHORS Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and Mizunoe,Y. TITLE Direct Submission JOURNAL Submitted (14-SEP-2015) to the DDBJ/EMBL/GenBank databases. Contact:Tadayuki Iwase Jikei University School of Medicine, Bacteriology; 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan REFERENCE 2 AUTHORS Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and Mizunoe,Y. TITLE Complete genome sequence of Klebsiella pneumoniae YH43 JOURNAL Unpublished (2015) COMMENT The genome sequence of K. pneumoniae strain YH43 was determined using 454 GS FLX Titanium system. A total of 524,416 single-end reads (218 Mb) and 142,440 8-kb paired-end reads (46 Mb) were assembled with GS Assembler software version 2.6 into one scaffold containing 58 gaps. The finishing was first performed by in silico analysis using GenoFinisher software (http://www.ige.tohoku.ac.jp/joho/gf_e/) and then remaining gaps were closed by sequencing of gap-spanning PCR products using an ABI3130xl DNA sequencer. To correct sequence error by resequencing of YH43 genome, a paired-end library of YH43 was constructed using the TruSeq DNA Sample Prep kit and sequenced by the Illumina MiSeq platform (2x150 bp). The mapping of obtained 2,085,021 reads (626 Mb) to YH43 genome sequence and the SNPs-calling were performed using BWAand SAMtools, respectively. Total 38 in-dels were corrected by the resequencing. The gene identification and annotation were conducted by the Microbial Genome Annotation Pipeline (MiGAP). ##Genome-Assembly-Data-START## Assembly Method :: GS Assembler software v. 2.6 Genome Coverage :: 40x Sequencing Technolog :: 454 GS FLX; Illumina MiSeq ##Genome-Assembly-Data-END## FEATURES Qualifiers source /db_xref="taxon:573" /mol_type="genomic DNA" /organism="Klebsiella pneumoniae" /strain="YH43" protein /locus_tag="KPYH43_c0647" /transl_table=11 BEGIN 1 MERVSITERP DWREKATEYG FNFHTMYGEP YWSEEAYYKL TLAQVEKLEE VTAELHQMCL 61 QVVEKVIASD ELMAKFRIPK HTWGFVRQSW KTNQPSLYSR LDLAWDGVGE PKLLENNADT 121 PTSLYEAAFF QWIWLEDQLN AGKLPAGSDQ FNSLQEKLIE RFGELREQFG FQLLHMACCR 181 DTVEDRGTVQ YLQDCAAEAG LATEFLYIED IGLGEKGQFT DLQDQVIGNL FKLYPWEFML 241 REMFSTKLED AGVRWLEPAW KSIISNKALL PMLWEMFPNH PNLLAAYFSE DAHPEMEKYV 301 IKPIFSREGA NVSIVEQGKV VESVEGPYGE EGTIVQEFYP LPKFGDSYTL IGSWLINDQP 361 AGIGIREDRA LITQDLSRFY PHIFVE //