LOCUS       BAS32991.1               397 aa    PRT              BCT 15-SEP-2015
DEFINITION  Klebsiella pneumoniae 2-amino-3-ketobutyrate coenzyme
            A ligase protein.
ACCESSION   AP014950-131
PROTEIN_ID  BAS32991.1
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5520319)
  AUTHORS   Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and
            Mizunoe,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2015) to the DDBJ/EMBL/GenBank databases.
            Contact:Tadayuki Iwase
            Jikei University School of Medicine, Bacteriology; 3-25-8
            Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan
REFERENCE   2
  AUTHORS   Iwase,T., Ogura,Y., Ishiwata,K., Hayashi,T., Yoneda,M. and
            Mizunoe,Y.
  TITLE     Complete genome sequence of Klebsiella pneumoniae YH43
  JOURNAL   Unpublished (2015)
COMMENT     The genome sequence of K. pneumoniae strain YH43 was determined
            using 454 GS FLX Titanium system. A total of 524,416 single-end
            reads (218 Mb) and 142,440 8-kb paired-end reads (46 Mb) were
            assembled with GS Assembler software version 2.6 into one scaffold
            containing 58 gaps. The finishing was first performed by in silico
            analysis using GenoFinisher software 
            (http://www.ige.tohoku.ac.jp/joho/gf_e/) and then remaining gaps
            were closed by sequencing of gap-spanning PCR products using an
            ABI3130xl DNA sequencer. To correct sequence error by resequencing
            of YH43 genome, a paired-end library of YH43 was constructed using
            the TruSeq DNA Sample Prep kit and sequenced by the Illumina MiSeq
            platform (2x150 bp). The mapping of obtained 2,085,021 reads (626
            Mb) to YH43 genome sequence and the SNPs-calling were performed
            using BWAand SAMtools, respectively. Total 38 in-dels were
            corrected by the resequencing. The gene identification and
            annotation were conducted by the Microbial Genome Annotation
            Pipeline (MiGAP).
            
            ##Genome-Assembly-Data-START##
            Assembly Method      :: GS Assembler software v. 2.6
            Genome Coverage      :: 40x
            Sequencing Technolog :: 454 GS FLX; Illumina MiSeq
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:573"
                     /mol_type="genomic DNA"
                     /organism="Klebsiella pneumoniae"
                     /strain="YH43"
     protein         /locus_tag="KPYH43_c0131"
                     /transl_table=11
BEGIN
        1 MRGDFYKQLT SNLETARAEG LFKEERIITS AQQADITVGD SHVINFCANN YLGLANHPEL
       61 IAAAKSGMDS HGFGMASVRF ICGTQDTHKQ LEKKLADFLG MEDAILYSSC FDANGGLFET
      121 LLGPEDAIIS DALNHASIID GVRLCKAKRF RYANNDMQEL EARLKEAREA GARHVLIATD
      181 GVFSMDGVIA NLKGVCDLAD KYDALVMVDD SHAVGFVGEN GRGSHEYCDV MGRVDIITGT
      241 LGKALGGASG GYTAARKEVV EWLRQRSRPY LFSNSLAPAI VAASIKVLEM VEEGADLRDR
      301 LWANARLFRE KMTAAGFTLA GADHAIIPVM LGEAVVAQSF ARELQKEGIY VTGFFYPVVP
      361 KGQARIRTQM SAAHTPEQIE RAVEAFTRIG KQLGVIA
//