LOCUS       BAL40520.1               765 aa    PRT              BCT 04-JUN-2019
DEFINITION  Escherichia coli str. K-12 substr. MDS42 predicted formate
            acetyltransferase 2 protein.
ACCESSION   AP012306-3200
PROTEIN_ID  BAL40520.1
SOURCE      Escherichia coli str. K-12 substr. MDS42
  ORGANISM  Escherichia coli str. K-12 substr. MDS42
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976195)
  AUTHORS   Ying,B.W., Seno,S., Suzuki,S. and Yomo,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2011) to the DDBJ/EMBL/GenBank databases.
            Contact:Shigeto Seno
            Osaka University, Graduate School of Information Science and
            Technology; Yamadaoka 1-5, Suita, Osaka 565-0871, Japan
REFERENCE   2
  AUTHORS   Fredens,J., Wang,K., de la Torre,D., Funke,L.F.H., Robertson,W.E.,
            Christova,Y., Chia,T., Schmied,W.H., Dunkelmann,D.L., Beranek,V.,
            Uttamapinant,C., Llamazares,A.G., Elliott,T.S. and Chin,J.W.
  TITLE     Total synthesis of Escherichia coli with a recoded genome
  JOURNAL   Nature 569, 514-518 (2019)
  REMARK    DOI:10.1038/s41586-019-1192-5
COMMENT     MDS42 is a genome reduced strain constructed from MG1655 by Posfai
            et al (originally reported in Science 2006), and is commercially
            available from Scarab Genomics (the founder is Dr. Frederick R.
            Blattner). We re-confirmed the whole genome sequence of MDS42 by
            the next generation resequencing technology based on SOLiD3
            (Applied Biosystems)
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Bioscope v. 1.3
            Genome Coverage       :: 300x
            Sequencing Technology :: SOLiD 3
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /db_xref="taxon:1110693"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli str. K-12 substr. MDS42"
                     /strain="K-12"
                     /sub_strain="MDS42"
     protein         /gene="pflD"
                     /gene_synonym="ECK3942"
                     /gene_synonym="JW3923"
                     /gene_synonym="b3951"
                     /locus_tag="ECMDS42_3388"
                     /note="pyruvate formate lyase II"
                     /transl_table=11
BEGIN
        1 MTNRISRLKT ALFANTREIS LERALLYTAS HRQTEGEPVI LRRAKATAYI LEHVEISIRD
       61 EELIAGNRTV KPRAGIMSPE MDPYWLLKEL DQFPTRPQDR FAISEEDKRI YREELFPYWE
      121 KRSMKDFING QMTDEVKAAT NTQIFSINQT DKGQGHIIID YPRLLNHGLG ELVAQMQQHC
      181 QQQPENHFYQ AALLLLEASQ KHILRYAELA ETMAANCTDA QRREELLTIA EISRHNAQHK
      241 PQTFWQACQL FWYMNIILQY ESNASSLSLG RFDQYMLPFY QTSLTQGEDA AFLKELLESL
      301 WVKCNDIVLL RSTSSARYFA GFPTGYTALL GGLTENGRSA VNVLSFLCLD AYQSVQLPQP
      361 NLGVRTNALI DTPFLMKTAE TIRFGTGIPQ IFNDEVVVPA FLNRGVSLED ARDYSVVGCV
      421 ELSIPGRTYG LHDIAMFNLL KVMEICLHEN EGNAALTYEG LLEQIRAKIS HYITLMVEGS
      481 NICDIGHRDW APVPLLSSFI SDCLEKGRDI TDGGARYNFS GVQGIGIANL SDSLHALKGM
      541 VFEQQRLSFD ELLSVLKANF ATPEGEKVRA RLINRFEKYG NDIDEVDNIS AELLRHYCKE
      601 VEKYQNPRGG YFTPGSYTVS AHVPLGSVVG ATPDGRFAGE QLADGGLSPM LGQDAQGPTA
      661 VLKSVSKLDN TLLSNGTLLN VKFTPATLEG EAGLRKLADF LRAFTQLKLQ HIQFNVVNAD
      721 TLREAQQRPQ DYAGLVVRVA GYSAFFVELS KEIQDDIIRR TAHQL
//