LOCUS BAL37379.1 232 aa PRT BCT 04-JUN-2019 DEFINITION Escherichia coli str. K-12 substr. MDS42 thiamin transporter subunit protein. ACCESSION AP012306-59 PROTEIN_ID BAL37379.1 SOURCE Escherichia coli str. K-12 substr. MDS42 ORGANISM Escherichia coli str. K-12 substr. MDS42 Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (bases 1 to 3976195) AUTHORS Ying,B.W., Seno,S., Suzuki,S. and Yomo,T. TITLE Direct Submission JOURNAL Submitted (30-NOV-2011) to the DDBJ/EMBL/GenBank databases. Contact:Shigeto Seno Osaka University, Graduate School of Information Science and Technology; Yamadaoka 1-5, Suita, Osaka 565-0871, Japan REFERENCE 2 AUTHORS Fredens,J., Wang,K., de la Torre,D., Funke,L.F.H., Robertson,W.E., Christova,Y., Chia,T., Schmied,W.H., Dunkelmann,D.L., Beranek,V., Uttamapinant,C., Llamazares,A.G., Elliott,T.S. and Chin,J.W. TITLE Total synthesis of Escherichia coli with a recoded genome JOURNAL Nature 569, 514-518 (2019) REMARK DOI:10.1038/s41586-019-1192-5 COMMENT MDS42 is a genome reduced strain constructed from MG1655 by Posfai et al (originally reported in Science 2006), and is commercially available from Scarab Genomics (the founder is Dr. Frederick R. Blattner). We re-confirmed the whole genome sequence of MDS42 by the next generation resequencing technology based on SOLiD3 (Applied Biosystems) ##Genome-Assembly-Data-START## Assembly Method :: Bioscope v. 1.3 Genome Coverage :: 300x Sequencing Technology :: SOLiD 3 ##Genome-Assembly-Data-END## FEATURES Qualifiers source /db_xref="taxon:1110693" /mol_type="genomic DNA" /organism="Escherichia coli str. K-12 substr. MDS42" /strain="K-12" /sub_strain="MDS42" protein /gene="thiQ" /gene_synonym="ECK0067" /gene_synonym="JW0065" /gene_synonym="b0066" /locus_tag="ECMDS42_0059" /transl_table=11 BEGIN 1 MLKLTDITWL YHHLPMRFSL TVERGEQVAI LGPSGAGKST LLNLIAGFLT PASGSLTIDG 61 VDHTTMPPSR RPVSMLFQEN NLFSHLTVAQ NIGLGLNPGL KLNAVQQGKM HAIARQMGID 121 NLMARLPGEL SGGQRQRVAL ARCLVREQPI LLLDEPFSAL DPALRQEMLT LVSTSCQQQK 181 MTLLMVSHSV EDAARIATRS VVVADGRIAW QGMTNELLSG KASASALLGI TG //