LOCUS BAE72953.1 149 aa PRT PRI 06-OCT-2006 DEFINITION Macaca fascicularis hypothetical protein protein. ACCESSION AB220420-1 PROTEIN_ID BAE72953.1 SOURCE Macaca fascicularis (crab-eating macaque) ORGANISM Macaca fascicularis Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Macaca. REFERENCE 1 (bases 1 to 1837) AUTHORS Hashimoto,K., Osada,N. and Kusuda,J. TITLE Direct Submission JOURNAL Submitted (04-JUL-2005) to the DDBJ/EMBL/GenBank databases. Contact:Katsuyuki Hashimoto National Institute of Biomedical Innovation, Division of Biomedical Resources; 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085, Japan REFERENCE 2 AUTHORS Kobayashi,M., Tanuma,R., Hirata,M., Osada,N., Kusuda,J., Sugano,S. and Hashimoto,K. TITLE Analysis of gene expression in cynomolgus monkey tissues by macaque cDNA oligo-chips JOURNAL Unpublished (2005) COMMENT Lab host: TOP10 Vector: pME18S-FL3 (Acc.No. AB009864) R. Site1: DraIII (CACTGTGTG) R. Site2: DraIII (CACCATGTG) Description: 1st strand cDNA was primed with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was synthesized using specific 5' and 3' primers and amplified by PCR. The PCR product was digested with SfiI and size selection was performed to exclude fragments <1.5kb.The SfiI-digested PCR product was cloned into distinct DraIII sites of pME18S-FL3. XhoI sites just outside the DraIII sites can be used to isolate the cDNA insert. Libraries were constructed by oligo-capping method. Libraries were made from: QccE: cerebellum cortex QnpA: parietal lobe QtrA: temporal lobe right QflA: frontal lobe left QmoA: medulla oblongata QbsA(B): brain stem QorA: occipital lobe right QtsA: testis Custom primers were used for 5' and 3'-end sequencing. The full-insert sequencing was done by primer-walking method using ABI DNA sequencer. FEATURES Qualifiers source /clone="QccE-14817" /clone_lib="macaque brain cDNA library QccE" /db_xref="taxon:9541" /dev_stage="adult" /mol_type="mRNA" /organism="Macaca fascicularis" /sex="male" /tissue_type="cerebellum cortex" protein /note="similar to Homo sapiens calmodulin 1 (phosphorylase kinase, delta) (CALM1), mRNA, NM_006888.2." /transl_table=1 BEGIN 1 MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG 61 NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE 121 EVDEMIREAD IDGDGQVNYE EFVQMMTAK //