LOCUS BAD95523.1 460 aa PRT BCT 07-OCT-2016 DEFINITION Rhodococcus opacus B4 benzene dioxygenase large subunit protein. ACCESSION AP011117-158 PROTEIN_ID BAD95523.1 SOURCE Rhodococcus opacus B4 ORGANISM Rhodococcus opacus B4 Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae; Rhodococcus. REFERENCE 1 (bases 1 to 244997) AUTHORS Sekine,M., Hosoyama,A., Kosugi,H., Yamada,R., Matsuo,Y., Tsukatani,N., Fujisawa,T., Takarada,H., Omata,S., Kishi,E., Shimizu,A., Koga,C., Saito,S., Nakazawa,H., Sekigawa,T., Nishiko,R., Takeyama,M., Tanikawa,S., Tago,S., Fujita,N., Kato,J., Na,K.S., Ohtake,H. and Harayama,S. TITLE Direct Submission JOURNAL Submitted (30-MAR-2009) to the DDBJ/EMBL/GenBank databases. Contact:Director-General Department of Biotechnology National Institute of Technology and Evaluation, NITE Genome Analysis Center (NGAC), Department of Biotechnology; 2-49-10 Nishihara, Shibuya-ku, Tokyo 151-0066, Japan URL :http://www.bio.nite.go.jp/ REFERENCE 2 AUTHORS Takarada,H., Sekine,M., Hosoyama,A., Yamada,R., Fujisawa,T., Omata,S., Shimizu,A., Tsukatani,N., Tanikawa,S., Fujita,N. and Harayama,S. TITLE Comparison of the complete genome sequences of Rhodococcus erythropolis PR4 and Rhodococcus opacus B4 JOURNAL Unpublished (2009) REFERENCE 3 AUTHORS Na,K.S., Kuroda,A., Takiguchi,N., Ikeda,T., Ohtake,H. and Kato,J. TITLE Isolation and characterization of benzene-tolerant Rhodococcus opacus strains. JOURNAL J. Biosci. Bioeng. 99, 378-382 (2005) COMMENT Kato,J., Na,K. and Ohtake,H. are at Department of Molecular Biotechnology, Hiroshima University. Rhodococcus opacus B-4 strain was isolated as a benzene-tolerant bacterium (Kyung-su Na et al 2005). The complete genome analysis of R. opacus B-4 was carried out by NITE. The genome analysis indicated that this strain has one linear chromosome, two linear plasmids pROB01, pROB02, and three circular plasmids pKNR, pKNR01, pKNR02. Partial nucleotide sequence of the linear plasmid pROB02 was previously determined by Kato et al, and was registered with the accession number AB193045, which is now merged into this record with the permission of Dr. Kato. The differences between the entry AB193045 and this record are as follows. 'C' at position 169867 was inserted. 'GC' at position 170058-170059 was inserted. 'T' at position 170676 replaced 'C'. 'T' at position 171855 replaced 'G'. 'GCT' at position 173294-173296 replaced 'CTG'. 'A' at position 173783 replaced 'C'. 'AT' at position 173819-173820 was inserted. 'CCT' at position 174725-174727 replaced 'GCC'. This work was supported by New Energy and Industrial Technology Development Organization (NEDO). Please visit our web site. URL:http://www.bio.nite.go.jp/ DOGAN ; Database of Genomes Analyzed at NITE The database contains genome sequence data, physical maps and the genomic and proteomic data of the microorganisms analyzed. URL:http://www.bio.nite.go.jp/dogan/Top The microbial genomic DNA clones used for the sequencing project are available through the NBRC. URL:http://www.nbrc.nite.go.jp/e/index.html FEATURES Qualifiers source /db_xref="taxon:632772" /mol_type="genomic DNA" /organism="Rhodococcus opacus B4" /plasmid="pROB02" /strain="B4" protein /EC_number="1.14.12.3" /gene="bnzA1" /locus_tag="ROP_pROB02-01580" /transl_table=11 BEGIN 1 MTDVQCEPAL AGRKPKWADA DIAELVDERT GRLDPRIYTD EALYEQELER IFGRSWLLMG 61 HETQIPKAGD FMTNYMGEDP VMVVRQKNGE IRVFLNQCRH RGMRICRADG GNAKSFTCSY 121 HGWAYDTGGN LVSVPFEEQA FPGLRKEDWG PLQARVETYK GLIFANWDAD APDLDTYLGE 181 AKFYMDHMLD RTEAGTEAIP GIQKWVIPCN WKFAAEQFCS DMYHAGTTSH LSGILAGLPD 241 GVDLSELAPP TEGIQYRATW GGHGSGFYIG DPNLLLAIMG PKVTEYWTQG TAAEKASERL 301 GSTERGQQLM AQHMTIFPTC SFLPGINTIR AWHPRGPNEI EVWAFTVVDA DAPEEMKEEY 361 RQQTLRTFSA GGVFEQDDGE NWVEIQQVLR GHKARSRPFN AEMGLGQTDT DNPDYPGTIS 421 YVYSEEAARG LYTQWVRMMT SPDWAALDAT RPAVSESTHT //