LOCUS AZN95481.1 399 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa glutathione-independent formaldehyde dehydrogenase protein. ACCESSION CP032541-5683 PROTEIN_ID AZN95481.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, wapR, and aroA JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Assembly Name :: PGN5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 217.64x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 16:28:44 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,903 CDS (total) :: 5,840 Genes (coding) :: 5,779 CDS (coding) :: 5,779 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 12 of 61 Pseudo Genes (frameshifted) :: 22 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN5" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="21-Nov-2017" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-" protein /locus_tag="PGN5_28730" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_254108.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MSGNRGVVYL GPGKVEVQNI PYPKMQDPQG RQIDHGVILR VVSTNICGSD QHMVRGRTTA 61 PEGLVLGHEI TGEVVEIGRG VETMKIGDLV SVPFNVACGH CRTCKEQHTG VCLTVNPARA 121 GGAYGYVDMG GWVGGQAEYV LVPYADFNLL KLPNREAAME KIRDLTCLSD ILPTGYHGAV 181 TAGVGPGSTV YIAGAGPVGL AAAASARLLG AAVVIVGDVN PTRLAHAKKQ GFEIADLSKD 241 TPLHEQIAAL LGEPEVDCAV DAVGFEARGH GHSGSQQEAP ATVLNSLMGI TRVAGKIGIP 301 GLYVTEDPGA VDAAAKHGAL SIRFGLGWAK SHSFHTGQTP VMKYNRQLMQ AIMWDRIKIA 361 DIVGVEVITL DDAPKGYGEF DAGVPKKFVI DPHNLFRAA //