LOCUS AZN93536.1 268 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa cyclohexadienyl dehydratase protein. ACCESSION CP032541-3661 PROTEIN_ID AZN93536.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, wapR, and aroA JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Assembly Name :: PGN5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 217.64x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 16:28:44 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,903 CDS (total) :: 5,840 Genes (coding) :: 5,779 CDS (coding) :: 5,779 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 12 of 61 Pseudo Genes (frameshifted) :: 22 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN5" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="21-Nov-2017" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-" protein /locus_tag="PGN5_18500" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_252165.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MPKSFRHLVQ ALACLALLAS ASLQAQESRL DRILESGVLR VATTGDYKPF SYRTEEGGYA 61 GFDVDMAQRL AESLGAKLVV VPTSWPNLMR DFADDRFDIA MSGISINLER QRQAYFSIPY 121 LRDGKTPITL CSEEARFQTL EQIDQPGVTA IVNPGGTNEK FARANLKKAR ILVHPDNVTI 181 FQQIVDGKAD LMMTDAIEAR LQSRLHPELC AVHPQQPFDF AEKAYLLPRD EAFKRYVDQW 241 LHIAEQSGLL RQRMEHWLEY RWPTAHGK //