LOCUS AZN90382.1 376 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa sensor domain-containing diguanylate cyclase protein. ACCESSION CP032541-359 PROTEIN_ID AZN90382.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, wapR, and aroA JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Assembly Name :: PGN5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 217.64x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 16:28:44 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,903 CDS (total) :: 5,840 Genes (coding) :: 5,779 CDS (coding) :: 5,779 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 12 of 61 Pseudo Genes (frameshifted) :: 22 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN5" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="21-Nov-2017" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-" protein /locus_tag="PGN5_01800" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_249029.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MRRLRFPRKR IDCLPFGRGH WPVERARDCR KMDERASKPP GLLPLSREDL EKTLRYALEI 61 VSDGIWDWNI ATNQVSRSAG WYLMLGYPPH SLPESVETWK SIIHPEDYPR VMASFQAYLD 121 GESPEYCEEY RCRTYSGDYL WISDRGRFVE FDERGEPRRM IGAHHEIHQR KLVELELQQR 181 NEELFDWNLR LEELVAERTE ALHRVNQALA SKMAEAQRLS EIDPLTELYN RRKFEQCLHH 241 EWMRRQRHGR ATALVMIDVD HFKRINDLFG HSTGDRVLVA FGRLVASELR EVDVLARWGG 301 EEFILLLPET GLEAAAALAE RLRQRVRSQS FEMGERLTAS FGVGVLNDGE TLDLLLCRVD 361 DALYRAKQRR DCVACC //