LOCUS AZN90340.1 452 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa glutamine synthetase protein. ACCESSION CP032541-315 PROTEIN_ID AZN90340.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, wapR, and aroA JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Assembly Name :: PGN5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 217.64x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 16:28:44 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,903 CDS (total) :: 5,840 Genes (coding) :: 5,779 CDS (coding) :: 5,779 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 12 of 61 Pseudo Genes (frameshifted) :: 22 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN5" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="21-Nov-2017" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-" protein /locus_tag="PGN5_01580" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_248989.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MTTKLDQLTS WLKERKITEV ECLISDLTGI ARGKISPTNK FIAEKGMRLP ESVLLQTVTG 61 DYVEDDIYYD LLDPADIDMV CRPDENAVFL VPWAIEPTAM VIHDTFDKLG NPIELSPRNI 121 LKRVLKMYAD KGWRPIVAPE MEFYLTKRSD DPDYPLQAPV GRSGRQETGR QSFSIDAANE 181 FDPLFEDMYD WCEAQGLDLD TLIHEEGTAQ MEINFRHGDA LDLADQILVF KRTMREAALK 241 HNVAATFMAK PMTGEPGSAM HLHQSIVDVK TGKNIFSNAD GTMSELFLHH IGGLQKFIPE 301 VLPLFAPNVN SFRRFLPDTS APVNVEWGEE NRTVGLRVPD SSPENRRVEN RLAGADANPY 361 LALAASLLCG YIGMVEGIKP SAQVKGRGYE RRNLRLPLTI EAALERMENC KPLEQYLGSK 421 FISGYVAVKR AEHENFKRVI SSWEREFLLL SV //