LOCUS AZN90253.1 554 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa malonate decarboxylase subunit alpha protein. ACCESSION CP032541-223 PROTEIN_ID AZN90253.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, wapR, and aroA JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Assembly Name :: PGN5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 217.64x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 16:28:44 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,903 CDS (total) :: 5,840 Genes (coding) :: 5,779 CDS (coding) :: 5,779 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 12 of 61 Pseudo Genes (frameshifted) :: 22 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN5" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="21-Nov-2017" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-" protein /gene="mdcA" /locus_tag="PGN5_01115" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_248899.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MTTPISPPPQ WSRRRQEKQR RLERVRGLAD GAVLPREGLV AALEALIAPG DRVVLEGNNQ 61 KQADFLSRSL ARVDPGKLHD LHMIMPSVGR PEHLDLFELG IARKLDFSFS GPQSLRIGQL 121 LEDGLLEIGA IHTYIELYAR LVVDLIPNVA LVAGFVADRE GNVYTGPSTE DTPALVEPTA 181 FSDGIVIVQV NRIVDDPRDL PRVDIPASWV DFVVEADQPF YIEPLFTRDP RHIKPVHVLM 241 AMMAIRGIYQ RHNVQSLNHG IGFNTAAIEL ILPTYGESLG LKGKICRHWT LNPHPTLIPA 301 IESGWVESVH CFGTELGMEG YIAQRPDVFF TGRDGSLRSN RMFCQLAGQY AVDLFIGATL 361 QVDGDGHSST VTRGRLAGFG GAPNMGHDPR GRRHSTPAWL DMRGEPEALL ERGRKLVVQM 421 VETFQDGGKP TFVERLDALE VARQTGMPLA PVMIYGDDVT HVLTEEGIAY LYKARSLEER 481 QAMIAAVAGI SPIGLRHDPR ETQRMRREGL IALPEDLGIR RTDASRELLA AKSIAELVEW 541 SGGLYQPPAR FRSW //