LOCUS AZN90100.1 610 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa asparagine synthase (glutamine- hydrolyzing) protein. ACCESSION CP032541-61 PROTEIN_ID AZN90100.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, wapR, and aroA JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Assembly Name :: PGN5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 217.64x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 16:28:44 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,903 CDS (total) :: 5,840 Genes (coding) :: 5,779 CDS (coding) :: 5,779 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 12 of 61 Pseudo Genes (frameshifted) :: 22 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN5" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="21-Nov-2017" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-" protein /gene="asnB" /locus_tag="PGN5_00305" /EC_number="6.3.5.4" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_248741.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MCGLAGWVDY TRKLDDEFPA IFAMTDTLAL RGPDAEGIWK HRNALLGHRR LAVIDLSGGV 61 QPMSYRFPTG QEVTLVYTGE VYNHDALRER LRRAGHEFRT RSDTEVVLHA YLQWGERCCE 121 YLTGMFAFAV FDGRDGHLLL VRDRLGIKPL YYARHREGLL FGSEIKSILA HPEFAARLDA 181 VGLVDLLTLS RGTSQTPFRE VQELLPGHLL SWRPNSQAKL RRYWEVRRQE HADDLQSTVQ 241 RTRELVTRAL GAQLHADVPV CSLLSGGLDS TALTGIAQRI AKAEHGGDIN SFSVDFVGQA 301 EQFRSDDLRP DQDQPFALLA AQYIGSRHRT VLIDNAELVC ERAREEVFRA KDVPFTFGDM 361 DTSLHLMFGE IRRHSTVAIS GEGADELFGG YGWFRDPQAV AAARFPWASR VRLPAGFIDA 421 GFNRRCDLLQ YQQASYDDGL RQVEHLAGDS PEERRMREFS HLHLKRWMVL LLERKDRLSM 481 CNGLEVRVPY TDHELVEYVY NVPWSIKSRD GEEKWLLKRA CADYVPEAVL KRRKSPYPTS 541 ANLGYERFLR GSVRRLLEDA VNPVFGIVSR EFLAAELEHP EGYFNTQVSR HNLETALALE 601 GWLRLYGLSA //