LOCUS AZN90050.1 684 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa glycine--tRNA ligase subunit beta protein. ACCESSION CP032541-8 PROTEIN_ID AZN90050.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, wapR, and aroA JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264451) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Assembly Name :: PGN5 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 217.64x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 16:28:44 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,903 CDS (total) :: 5,840 Genes (coding) :: 5,779 CDS (coding) :: 5,779 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 12 of 61 Pseudo Genes (frameshifted) :: 22 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN5" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="21-Nov-2017" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-, aroA-" protein /locus_tag="PGN5_00040" /EC_number="6.1.1.14" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_064728.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MSAKDFLVEL GTEELPPKAL NSLGEAFLSG IEKGLKAAGL SYAAARFYAA PRRLAVLVEQ 61 LAVQQPDRTV NLDGPPLQAA FDASGNPTQA ALGFAKKCGV DLQQIDKSGP KLRFIQTIAG 121 QPAAGLLPGI VEASLNELPI PKRMRWAARR EEFVRPTQWL VMLFGDDVVE CEILAQKAGR 181 ESRGHRFHNP DNVRISSPAA YLEDLRGAHV LADFAERREL IAKRVAELAA EQQGSAIVPP 241 SLLDEVTALV EWPVPLVCSF EERFLEVPQE ALITTMQDNQ KYFCLLDANG KLLPRFITVA 301 NVESKAPENI VSGNEKVVRP RLTDAEFFFK QDKKQPLESF NERLRNVVFQ AQLGTVFEKA 361 QRVSGLAAYI AERIGGNAQN ASRAGILSKC DLATEMVGEF PEMQGIAGYY YATHGGEAED 421 VALALNEQYM PRGAGAELPS TLTGAAVAVA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL 481 GVLRILIEKQ LDLDLVAAVN AAVEQYGDKV KAAGLAEQVL DFVFDRLRAR YEDEGVDVAV 541 YQSVRALKPS SPLDFDQRVQ AVQAFRQLPE AEALAAANKR VSNILAKSED EVPPNVDASL 601 LVEAAEKALG SAVANAESEV APLAAARDYR AALARLAALR EPVDTFFADV MVNVDDAAVR 661 ANRYALLAKL RGSFLGVADI SLLG //