LOCUS       AZN89855.1               643 aa    PRT              BCT 18-DEC-2018
DEFINITION  Pseudomonas aeruginosa type VI secretion system tip protein
            VgrG protein.
ACCESSION   CP032540-100
PROTEIN_ID  AZN89855.1
SOURCE      Pseudomonas aeruginosa
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 6264422)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the
            following genes: toxA, plcH, phzM, and wapR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6264422)
  AUTHORS   Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory,
            PO Box 1663 M888, Los Alamos, NM 87545, USA
COMMENT     Bacteria and source DNA available from Dr. Hongwei Yu
            (yuh@marshall.edu).
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date             :: 20-SEP-2018
            Assembly Method           :: Bowtie2 v. 2018-09-20
            Genome Representation     :: Full
            Expected Final Version    :: Yes
            Reference-guided Assembly :: NC_002516.2
            Genome Coverage           :: 368.17x
            Sequencing Technology     :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/26/2018 12:47:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS+
            Annotation Software revision      :: 4.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 5,631
            CDS (total)                       :: 5,568
            Genes (coding)                    :: 5,507
            CDS (coding)                      :: 5,507
            Genes (RNA)                       :: 63
            rRNAs                             :: 4 (5S)
            complete rRNAs                    :: 4 (5S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            Pseudo Genes (ambiguous residues) :: 13 of 61
            Pseudo Genes (frameshifted)       :: 21 of 61
            Pseudo Genes (incomplete)         :: 18 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 4 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="PGN4"
                     /isolation_source="derived from laboratory strain"
                     /db_xref="taxon:287"
                     /country="USA: WV, Huntington"
                     /collection_date="13-Sep-2014"
                     /collected_by="Progenesis Technologies, LLC"
                     /identified_by="Progenesis Technologies, LLC"
                     /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-"
     protein         /gene="tssI"
                     /locus_tag="PGN4_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MQLTRLVQVD CPLGPDVLLL QRMEGREELG RLFAYELHLV SENPNLPLEQ LLGKPMSLSL
       61 ELPGGSRRFF HGIVARCSQV AGHGQFAGYQ ATLRPWPWLL TRTSDCRIFQ NQSVPEIIKQ
      121 VFRNLGFSDF EDALTRPYRE WEYCVQYRET SFDFISRLME QEGIYYWFRH EQKRHILVLS
      181 DAYGAHRSPG GYASVPYYPP TLGHRERDHF FDWQMAREVQ PGSLTLNDYD FQRPGARLEV
      241 RSNIARPHAA ADYPLYDYPG EYVQSQDGEQ YARNRIEAIQ AQHERVRLRG VVRGIGAGHL
      301 FRLSGYPRDD QNREYLVVGA EYRVVQELYE TGSGGAGSQF ESELDCIDAS QSFRLLPQTP
      361 VPVVRGPQTA VVVGPKGEEI WTDQYGRVKV HFHWDRHDQS NENSSCWIRV SQAWAGKNWG
      421 SMQIPRIGQE VIVSFLEGDP DRPIITGRVY NAEQTVPYEL PANATQSGMK SRSSKGGTPA
      481 NFNEIRMEDK KGAEQLYIHA ERNQDNLVEN DASLSVGHDR NKSIGHDELA RIGNNRTRAV
      541 KLNDTLLVGG AKSDSVTGTY LIEAGAQIRL VCGKSVVEFN ADGTINISGS AFNLYASGNG
      601 NIDTGGRLDL NSGGASEVDA KGKGVQGTID GQVQAMFPPP AKG
//