LOCUS AZN84979.1 425 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa NAD-dependent dihydropyrimidine dehydrogenase subunit PreA protein. ACCESSION CP032540-463 PROTEIN_ID AZN84979.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, and wapR JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu (yuh@marshall.edu). Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 368.17x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 12:47:58 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,631 CDS (total) :: 5,568 Genes (coding) :: 5,507 CDS (coding) :: 5,507 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 13 of 61 Pseudo Genes (frameshifted) :: 21 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN4" /isolation_source="derived from laboratory strain" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="13-Sep-2014" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-" protein /gene="preA" /locus_tag="PGN4_02320" /EC_number="1.3.1.1" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_249130.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MMADLSIEFA GIKSPNPFWL ASAPPTDKAY NVVRAYQAGW GGVVWKTLGE DPAAVNVSSR 61 YSAHFGPNRQ VQGINNIELI TDRSLEINLR EIAQVKKDWP DRALIVSLMV PCVEESWKYI 121 LPLVEATGAD GIELNFGCPH GMPERGMGAA VGQVPEYVEM VTRWCKAHCS LPVIVKLTPN 181 ITDIRQSARA AHRGGADAVS LINTINSITS VDLERMVALP VVGTQSTHGG YCGSAVKPIA 241 LNMVAEIARD PQTRGLPICG IGGIGSWRDA AEFVALGCGA VQVCTAAMLH GFRIVEDMQD 301 GLARWMDSHG YQRLSDFSGG AVGNTTDWKY LDINYQVIAR IDQDACIGCG RCHIACEDTS 361 HQAIASLPRA DGTHRYEVID AECVGCNLCQ ITCPVENCIE MVPQDTGKPY LNWTQDPRNP 421 YREAS //