LOCUS AZN84933.1 344 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa twitching motility protein PilT protein. ACCESSION CP032540-417 PROTEIN_ID AZN84933.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, and wapR JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu (yuh@marshall.edu). Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 368.17x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 12:47:58 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,631 CDS (total) :: 5,568 Genes (coding) :: 5,507 CDS (coding) :: 5,507 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 13 of 61 Pseudo Genes (frameshifted) :: 21 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN4" /isolation_source="derived from laboratory strain" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="13-Sep-2014" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-" protein /locus_tag="PGN4_02090" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003286254.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MDITELLAFS AKQGASDLHL SAGLPPMIRV DGDVRRINLP PLEHKQVHAL IYDIMNDKQR 61 KDFEEFLETD FSFEVPGVAR FRVNAFNQNR GAGAVFRTIP SKVLTMEELG MGEVFKRVSD 121 VPRGLVLVTG PTGSGKSTTL AAMLDYLNNT KYHHILTIED PIEFVHESKK CLVNQREVHR 181 DTLGFSEALR SALREDPDII LVGEMRDLET IRLALTAAET GHLVFGTLHT TSAAKTIDRV 241 VDVFPAEEKA MVRSMLSESL QSVISQTLIK KIGGGRVAAH EIMIGTPAIR NLIREDKVAQ 301 MYSAIQTGGS LGMQTLDMCL KGLVAKGLIS RENAREKAKI PENF //