LOCUS AZN84908.1 495 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa insulinase family protein protein. ACCESSION CP032540-392 PROTEIN_ID AZN84908.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, and wapR JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu (yuh@marshall.edu). Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 368.17x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 12:47:58 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,631 CDS (total) :: 5,568 Genes (coding) :: 5,507 CDS (coding) :: 5,507 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 13 of 61 Pseudo Genes (frameshifted) :: 21 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN4" /isolation_source="derived from laboratory strain" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="13-Sep-2014" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-" protein /locus_tag="PGN4_01965" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_249062.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MSERTGLRYG LLGLIVIALL AALAYFVARP PQTPAAAHAA AVEENQLQSL KDVAKQAPVH 61 RKLDIQEWKT AEGAKVLFVE AHELPMFDLR LTFAAGSSQD AGTPGLSMLT NAMLNEGVPG 121 KDTTAIAAGF EDLGASFSNG SYRDMAVAGL RSLSEADKRT QALKLFEQVI GQPTFPEDAL 181 ARIKNQVLAG FEYQKQNPGK LAGLELFKRL YGEHPYAHSS DGDEKSVPTI SREQLQAFHK 241 KAYAAGNVVI ALVGDLSRQE AEAIAAEVSK ALPQGPALAK TVQPETPKPG LTHIDFPSEQ 301 THLMLAQLGI DRQDPDYAAL YLGNQILGGG GFGTRLMDQV REKRGLTYGI YSGFTAMQAR 361 GPFMINFQTR AELSEGALKL VQDIVRDYLA NGPTQKELDD AKRELAGSFP LSTASNADIV 421 GQLGAIGFYG LPLDYLESFL KQVEGLSVEQ VRTAMAKHLD ADAFVIVSAG PSVPQKPLPP 481 PTAKPAQQPS GVPEH //