LOCUS AZN84890.1 612 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa dihydroxy-acid dehydratase protein. ACCESSION CP032540-373 PROTEIN_ID AZN84890.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, and wapR JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu (yuh@marshall.edu). Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 368.17x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 12:47:58 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,631 CDS (total) :: 5,568 Genes (coding) :: 5,507 CDS (coding) :: 5,507 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 13 of 61 Pseudo Genes (frameshifted) :: 21 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN4" /isolation_source="derived from laboratory strain" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="13-Sep-2014" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-" protein /locus_tag="PGN4_01870" /EC_number="4.2.1.9" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003229166.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MPDYRSKTST HGRNMAGARA LWRATGMKDE DFKKPIIAIA NSFTQFVPGH VHLKDLGQLV 61 AREIEKAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSREII ADSVEYMVNA HCADAIVCIS 121 NCDKITPGML MAALRLNIPV VFVSGGPMEA GKTKLASHGL DLVDAMVVAA DDSCSDEKVA 181 EYERSACPTC GSCSGMFTAN SMNCLTEALG LSLPGNGSTL ATHADREQLF LRAGRLAVEL 241 CQRYYGEGDD SVLPRNIANF KAFENAMTLD IAMGGSTNTI LHLLAAAQEA EVPFDLRDID 301 RLSRKVPQLC KVAPNIQKYH MEDVHRAGGI FSILGELARG GLLHTDVPTV HSPSMADAIA 361 QWDITQTRDE AVHTFFKAGP AGIPTQTAFS QNTRWPSLDD DRAEGCIRSV EHAYSKEGGL 421 AVLYGNIALD GCVVKTAGVD ESIHVFEGSA KIFESQDAAV KGILGDEVKA GDIVIIRYEG 481 PKGGPGMQEM LYPTSYLKSK GLGKQCALLT DGRFSGGTSG LSIGHASPEA AAGGAIGLVQ 541 DGDKVLIDIP NRSINLLVSD EELAARRAEQ DKKGWKPAAP RARRVSTALK AYALLATSAD 601 KGAVRNKALL DG //