LOCUS AZN84617.1 444 aa PRT BCT 18-DEC-2018 DEFINITION Pseudomonas aeruginosa type VI secretion system baseplate subunit TssK protein. ACCESSION CP032540-88 PROTEIN_ID AZN84617.1 SOURCE Pseudomonas aeruginosa ORGANISM Pseudomonas aeruginosa Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Attenuated P. aeruginosa strain PAO1 with in-frame deletion of the following genes: toxA, plcH, phzM, and wapR JOURNAL Unpublished REFERENCE 2 (bases 1 to 6264422) AUTHORS Valentine,M., Withers,R.W., Johnson,S.L., Niles,R. and Yu,H. TITLE Direct Submission JOURNAL Submitted (25-SEP-2018) BioScience, Los Alamos National Laboratory, PO Box 1663 M888, Los Alamos, NM 87545, USA COMMENT Bacteria and source DNA available from Dr. Hongwei Yu (yuh@marshall.edu). Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Date :: 20-SEP-2018 Assembly Method :: Bowtie2 v. 2018-09-20 Genome Representation :: Full Expected Final Version :: Yes Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 368.17x Sequencing Technology :: Illumina NextSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 09/26/2018 12:47:58 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 4.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,631 CDS (total) :: 5,568 Genes (coding) :: 5,507 CDS (coding) :: 5,507 Genes (RNA) :: 63 rRNAs :: 4 (5S) complete rRNAs :: 4 (5S) tRNAs :: 55 ncRNAs :: 4 Pseudo Genes (total) :: 61 Pseudo Genes (ambiguous residues) :: 13 of 61 Pseudo Genes (frameshifted) :: 21 of 61 Pseudo Genes (incomplete) :: 18 of 61 Pseudo Genes (internal stop) :: 13 of 61 Pseudo Genes (multiple problems) :: 4 of 61 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa" /mol_type="genomic DNA" /strain="PGN4" /isolation_source="derived from laboratory strain" /db_xref="taxon:287" /country="USA: WV, Huntington" /collection_date="13-Sep-2014" /collected_by="Progenesis Technologies, LLC" /identified_by="Progenesis Technologies, LLC" /note="genotype: PAO1 toxA-, plcH-, phzM-, wapR-" protein /gene="tssK" /locus_tag="PGN4_00440" /inference="COORDINATES: similar to AA sequence:RefSeq:NP_248769.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MSWNNRVVWS EGMFLRPQHF QQHDRYLETL VDGRCRSLLA GGWGFSELKL DDALLTQGKL 61 AIVSARGVLP DGTPFNIPAD DPAPAPLNVE ESLRDGIVYL GLPLKRVGTR DTVEEGEALG 121 GARYVSQVQE VRDDNAAFES RAPVALGSQA FRLLTERDGL GEYAAVGVAR VREKRADQAL 181 SLDEDYLPPV LDIAAAPPLA SFAKELLGLL HQRGEALAGR VVASSAGGAS EIADFLLLQL 241 VNRAEALTGH LSRVRPLHPQ ELYRELVALA GEFCTFTASQ RRPEEYPVYN HDDLAASFAP 301 VMLALRQALA TVIDAKAIAI PIVEKAYGVH VAMLSDRSLI DNASFVLVVR ADVPGESLRG 361 HFPQQAKVGS VEHIRDLVNL QLPGIGLLPM PVAPRQIPYH AGSTYFELDR GSAHWKQLTH 421 SGGFAFHIAG QFPGLNLAFW AIRG //