LOCUS ASS93318.1 450 aa PRT BCT 08-AUG-2017
DEFINITION Peribacillus simplex NBRC 15720 = DSM 1321 UDP-N-acetylmuramoyl-
L-alanine--D-glutamate ligase protein.
ACCESSION CP017704-928
PROTEIN_ID ASS93318.1
SOURCE Peribacillus simplex NBRC 15720 = DSM 1321
ORGANISM Peribacillus simplex NBRC 15720 = DSM 1321
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae;
Peribacillus.
REFERENCE 1 (bases 1 to 5645783)
AUTHORS Park,M.-K., Lee,Y.-J., Yi,H., Bahn,Y.-S., Kim,J.F. and Lee,D.-W.
TITLE The whole genome sequencing and assembly of Bacillus simplex DSM
1321 strain
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5645783)
AUTHORS Park,M.-K., Lee,Y.-J., Yi,H., Bahn,Y.-S., Kim,J.F. and Lee,D.-W.
TITLE Direct Submission
JOURNAL Submitted (17-OCT-2016) Kyungpook National University, School of
Applied Biosciences, 80 Daehak-Ro, Bukgu, Daegu 41566, South Korea
COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (released 2013). Information about the Pipeline can be
found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
This genome has a base modification file available.
##Genome-Assembly-Data-START##
Assembly Method :: SMRT analysis 2.3 v. HGAP protocol 2
Genome Coverage :: missing
Sequencing Technology :: PacBio
##Genome-Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Date :: 10/17/2016 13:55:10
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline
Annotation Method :: Best-placed reference protein
set; GeneMarkS+
Annotation Software revision :: 3.3
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes (total) :: 5,348
CDS (total) :: 5,228
Genes (coding) :: 5,118
CDS (coding) :: 5,118
Genes (RNA) :: 120
rRNAs :: 13, 12, 12 (5S, 16S, 23S)
complete rRNAs :: 13, 12, 12 (5S, 16S, 23S)
tRNAs :: 77
ncRNAs :: 6
Pseudo Genes (total) :: 110
Pseudo Genes (ambiguous residues) :: 0 of 110
Pseudo Genes (frameshifted) :: 73 of 110
Pseudo Genes (incomplete) :: 32 of 110
Pseudo Genes (internal stop) :: 34 of 110
Pseudo Genes (multiple problems) :: 28 of 110
##Genome-Annotation-Data-END##
FEATURES Qualifiers
source /organism="Peribacillus simplex NBRC 15720 = DSM 1321"
/mol_type="genomic DNA"
/strain="DSM 1321"
/isolation_source="soil"
/type_material="type strain of Bacillus simplex"
/db_xref="taxon:1349754"
/collection_date="1989"
protein /gene="murD"
/locus_tag="BS1321_04655"
/inference="EXISTENCE: similar to AA
sequence:RefSeq:WP_003348886.1"
/note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
involved in peptidoglycan biosynthesis; cytoplasmic;
catalyzes the addition of glutamate to the nucleotide
precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
formation; Derived by automated computational analysis
using gene prediction method: Protein Homology."
/transl_table=11
BEGIN
1 MKETVKYSEK KILVLGLAKS GVMAASLLHK LGAFVTVNDM KPLSENPEAQ GLLEQGIKVI
61 CGSHPIELLD EGFELIVKNP GIPYRNPIVK GALEKGIPVI TEVELAYQIS EAPFIGITGT
121 NGKTTTTTLI YEMLKTGEKS PLIAGNIGTV ASGVAEKAKP DDSIVIELSS FQLMGIDEFR
181 PEISIVTNLY DAHLDYHSSK QEYVEAKAAI TKNQIASDFM IYNADQELVL SMAKKTKAEL
241 VPFSAEMKHE NGAYVEDGAV CFRGEKIIEV SEIALPGKHN LENVLAAISA AKLKGVANEA
301 IQTVLKTFYG VEHRLQFVAT IDNRKFYNDS KATNILAASK ALTSFTEPVI LLAGGLDRGN
361 EFDELKPAMK NVKAVITFGE TAEKIERVAK EIGIANIKRV DNVEKAVPAA FELSNEGDCI
421 LLSPACASWD QYKTFEQRGD MFMEAVHKLK
//