LOCUS       ASM95244.1               374 aa    PRT              BCT 14-AUG-2017
DEFINITION  Vibrio vulnificus NBRC 15645 = ATCC 27562 tRNA-specific
            2-thiouridylase protein.
ACCESSION   CP012881-197
PROTEIN_ID  ASM95244.1
SOURCE      Vibrio vulnificus NBRC 15645 = ATCC 27562
  ORGANISM  Vibrio vulnificus NBRC 15645 = ATCC 27562
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 3266118)
  AUTHORS   Rowe-Magnus,D. and Rusch,D.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Biology, Indiana University, 1001 E. Third
            Street, Bloomington, IN 47405, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method       :: Celera Assembler v. 8.1
            Genome Coverage       :: 10x; 100x
            Sequencing Technology :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 10/06/2015 12:48:27
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS+
            Annotation Software revision :: 3.0
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA;
                                            repeat_region
            Genes                        :: 4,415
            CDS                          :: 4,220
            Pseudo Genes                 :: 45
            rRNAs                        :: 12, 11, 11 (5S, 16S, 23S)
            complete rRNAs               :: 12, 11, 11 (5S, 16S, 23S)
            tRNAs                        :: 115
            ncRNA                        :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Vibrio vulnificus NBRC 15645 = ATCC 27562"
                     /mol_type="genomic DNA"
                     /strain="ATCC 27562"
                     /type_material="type strain of Vibrio vulnificus"
                     /db_xref="taxon:1219061"
                     /chromosome="1"
                     /country="USA"
                     /collection_date="1979"
     protein         /gene="mnmA"
                     /locus_tag="AOT11_08190"
                     /EC_number="2.8.1.-"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_013572026.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MSDNSQKKVI VGMSGGVDSS VSAYLLKQQG YQVEGLFMKN WEEDDDSEYC TAAEDLADAQ
       61 AVCDKLGIHL HKINFAAEYW DNVFEYFLSE YKAGRTPNPD ILCNKEIKFK AFLEFADEVL
      121 DADYIAMGHY VRRSFPENGE KPQMLRGLDG NKDQSYFLYT LSHEQVARSL FPVGELEKPE
      181 VRRIAEEQGL ITAKKKDSTG ICFIGERKFT DFLSRYLPAQ PGNIESPEGE VLGQHQGLMY
      241 HTLGQRKGLH IGGRKGGGGN EEPWFVAEKD LKRNVLIAVQ GQDHPMLKSE GLIASQLHWV
      301 EREPIRDVVK CTVKTRYRQQ DIPCTIIPID DENIKVIFDE PEIAVTPGQS AVFYQGDVCL
      361 GGGIIEKRIK YTQA
//