LOCUS ASM95244.1 374 aa PRT BCT 14-AUG-2017 DEFINITION Vibrio vulnificus NBRC 15645 = ATCC 27562 tRNA-specific 2-thiouridylase protein. ACCESSION CP012881-197 PROTEIN_ID ASM95244.1 SOURCE Vibrio vulnificus NBRC 15645 = ATCC 27562 ORGANISM Vibrio vulnificus NBRC 15645 = ATCC 27562 Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio. REFERENCE 1 (bases 1 to 3266118) AUTHORS Rowe-Magnus,D. and Rusch,D.B. TITLE Direct Submission JOURNAL Submitted (06-OCT-2015) Biology, Indiana University, 1001 E. Third Street, Bloomington, IN 47405, USA COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: Celera Assembler v. 8.1 Genome Coverage :: 10x; 100x Sequencing Technology :: PacBio; Illumina ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 10/06/2015 12:48:27 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 3.0 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes :: 4,415 CDS :: 4,220 Pseudo Genes :: 45 rRNAs :: 12, 11, 11 (5S, 16S, 23S) complete rRNAs :: 12, 11, 11 (5S, 16S, 23S) tRNAs :: 115 ncRNA :: 1 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Vibrio vulnificus NBRC 15645 = ATCC 27562" /mol_type="genomic DNA" /strain="ATCC 27562" /type_material="type strain of Vibrio vulnificus" /db_xref="taxon:1219061" /chromosome="1" /country="USA" /collection_date="1979" protein /gene="mnmA" /locus_tag="AOT11_08190" /EC_number="2.8.1.-" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_013572026.1" /note="catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs; Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MSDNSQKKVI VGMSGGVDSS VSAYLLKQQG YQVEGLFMKN WEEDDDSEYC TAAEDLADAQ 61 AVCDKLGIHL HKINFAAEYW DNVFEYFLSE YKAGRTPNPD ILCNKEIKFK AFLEFADEVL 121 DADYIAMGHY VRRSFPENGE KPQMLRGLDG NKDQSYFLYT LSHEQVARSL FPVGELEKPE 181 VRRIAEEQGL ITAKKKDSTG ICFIGERKFT DFLSRYLPAQ PGNIESPEGE VLGQHQGLMY 241 HTLGQRKGLH IGGRKGGGGN EEPWFVAEKD LKRNVLIAVQ GQDHPMLKSE GLIASQLHWV 301 EREPIRDVVK CTVKTRYRQQ DIPCTIIPID DENIKVIFDE PEIAVTPGQS AVFYQGDVCL 361 GGGIIEKRIK YTQA //