LOCUS APA94452.1 959 aa PRT BCT 30-JAN-2018 DEFINITION Nocardia seriolae DNA topoisomerase protein. ACCESSION CP017839-361 PROTEIN_ID APA94452.1 SOURCE Nocardia seriolae ORGANISM Nocardia seriolae Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Nocardia. REFERENCE 1 (bases 1 to 8304518) AUTHORS Han,H.-J. TITLE Genome sequence of Nocardia seriolae strain EM150506, isolated from Anguila japonica JOURNAL Unpublished REFERENCE 2 (bases 1 to 8304518) AUTHORS Han,H.-J. TITLE Direct Submission JOURNAL Submitted (21-OCT-2016) Pathology Research Division, National Institute of Fisheries Science, #216, Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 46083, South Korea COMMENT DNA/bacteria available from National Institute of Fisheries Science (NIFS) in South Korea. ##Genome-Assembly-Data-START## Assembly Method :: SMRT analysis v. 2.3.0 Genome Coverage :: 156x Sequencing Technology :: PacBio ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Nocardia seriolae" /mol_type="genomic DNA" /strain="EM150506" /host="Anguilla japonica" /db_xref="taxon:37332" /country="South Korea" /collection_date="2015-05-06" protein /gene="topA" /locus_tag="NS506_00369" /EC_number="5.99.1.2" /function="DNA-binding; Isomerase; Magnesium; Metal-binding; Topoisomerase." /note="Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone; Belongs to the type IA topoisomerase family; Contains 1 Toprim domain; KEGG: mei:Msip34_2877 DNA topoisomerase I" /transl_table=11 /db_xref="UniProtKB/Swiss-Prot:O51768" BEGIN 1 MIVESPTKAK KIAPYLGRGY VVEASVGHIR DLPRGAADVP AKYKGQQWAR LGVDVDHDFE 61 PIYVVSPEKK GKVSELKSLL ADADELYLAT DPDREGEAIA WHLLETLKPK VPVRRMVFHE 121 ITESAIRAAA ADTRELDQDL VDAQETRRIL DRLYGYEVSP VLWKKVMPRL SAGRVQSVAT 181 RVIVQRERER MAFRSAEYWD IAAKLDAGGN EEGGNPRVFG ARLVSVEGAR VATGRDFGSD 241 GQLKSQGVVV LDAGYAQRLA ESLHGVDLTV TSVESKPYTR KPYAPFMTST LQQEAARKLR 301 FTSERTMRVA QRLYENGYIT YMRTDSTTLS QSAINAARAQ ATQLYGAEYV SPTPRQYNRK 361 VKNAQEAHEA IRPSGDVFAT PGQLHNKIDN DEFRLYELIW QRTVASQMQD ARGTTLTVRI 421 TGVANSGEEC VFSASGRTIT FAGFLKAYVE SVDEEAGGQS DDAESRLPAL TEGQGVTAVE 481 LAPDGHTTNP PARFTEASLI KALEDLGIGR PSTYSSIIKT ILDRGYVYKR GSALVPSWVA 541 FAVIGLLEEH FGRLVDFDFT AAMEDDLDAI AGGREQRGNW LSSFYFGGDH GAQGSVARSG 601 GLKKMVGERL DGIDAREVNS IKLFADDEGR DVVVRVGKFG PYLERMIANP DDPDGNPVSQ 661 RANLPDDLPP DELTPEVAEK LFATPQEGRS LGTDPATGHE IVAKEGRFGP YVTEVLPEPA 721 EDIDTGAKKT AKKAADKPKP RTGSLFKSMS VETVTLDEAL KLLSLPRVVG VDPASGDEIT 781 AQNGRYGPYL KKGTDSRSLT GEDQIFSVTL EEALKIYAEP KRRGGQSASA PPLRELGVDP 841 VSEKPMVIKD GRFGPYVTDG ETNASLRKGD EVESITDARA SELLADRRAR GPVKKAAKKT 901 AKKAAAKKTA VAKTVVKKAA AKQAAAKTAD GVTTKKAAAA KKTPVKKAAA KKTAAAAKS //