LOCUS ANI08875.1 497 aa PRT BCT 02-JUN-2016 DEFINITION Pseudomonas aeruginosa SJTD-1 RNA polymerase factor sigma- 54 protein. ACCESSION CP015877-2086 PROTEIN_ID ANI08875.1 SOURCE Pseudomonas aeruginosa SJTD-1 ORGANISM Pseudomonas aeruginosa SJTD-1 Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 6243825) AUTHORS Liu,H., Liang,R., Tao,F., Ma,C., Liu,Y., Liu,X. and Liu,J. TITLE Genome sequence of Pseudomonas aeruginosa strain SJTD-1, a bacterium capable of degrading long-chain alkanes and crude oil JOURNAL J. Bacteriol. 194 (17), 4783-4784 (2012) PUBMED 22887679 REFERENCE 2 (bases 1 to 6243825) AUTHORS Liang,R., Tao,F., Liu,H. and Liu,J. TITLE Direct Submission JOURNAL Submitted (24-MAY-2012) State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, Shanghai 200240, China REFERENCE 3 (bases 1 to 6243825) AUTHORS Liang,R. TITLE Direct Submission JOURNAL Submitted (23-MAY-2016) State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, Shanghai 200240, China COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Source bacteria available from Dr. Rubing Liang (School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, China). ##Genome-Assembly-Data-START## Assembly Method :: ALLPATH-LG v. 2015; GapCloser v. 2015 Reference-guided Assembly :: NC_002516.2 Genome Coverage :: 200x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 05/25/2016 16:12:59 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 3.2 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,696 CDS (total) :: 5,617 Genes (coding) :: 5,603 CDS (coding) :: 5,603 Genes (RNA) :: 79 rRNAs :: 4, 4, 4 (5S, 16S, 23S) complete rRNAs :: 4, 4, 4 (5S, 16S, 23S) tRNAs :: 63 ncRNAs :: 4 Pseudo Genes (total) :: 14 Pseudo Genes (ambiguous residues) :: 0 of 14 Pseudo Genes (frameshifted) :: 5 of 14 Pseudo Genes (incomplete) :: 7 of 14 Pseudo Genes (internal stop) :: 3 of 14 Pseudo Genes (multiple problems) :: 1 of 14 CRISPR Arrays :: 3 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Pseudomonas aeruginosa SJTD-1" /mol_type="genomic DNA" /strain="SJTD-1" /isolation_source="soil" /db_xref="taxon:1193501" /country="China: Shanghai" protein /locus_tag="A214_10610" /inference="EXISTENCE: similar to AA sequence:SwissProt:P0A171.1" /note="sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; sigma 54 factor is responsible for the expression of enzymes involved in nitrogen assimilation and metabolism; the rhizobia often have 2 copies of this sigma factor; in Rhizobium etli RpoN1 shown to be involved in the assimilation of several nitrogen and carbon sources during free-living aerobic growth and RpoN2 is involved in symbiotic nitrogen fixation; in Bradyrhizobium both RpoN1 and N2 are functional in free-living and symbiotic conditions, rpoN1 gene was regulated in response to oxygen; Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ EDGDDFDNSD 61 PLADGAEQAA SAPQESPLQE SATPSVESLD DDQWSERIPS ELPVDTAWED IYQTSASSLP 121 SNDDDEWDFT ARTSSGESLH SHLLWQVNLA PMSDTDRMIA VTIIDSINND GYLEESLEEI 181 LAAIDPELDV ELDEVEVVLR RIQQLEPAGI GARNLRECLL LQLRQLPSTT PWLNEALRLV 241 SDYLDLLGGR DYSQLMRRMK LKEDELRQVI ELIQCLHPRP GSQIESSEAE YIVPDVIVRK 301 DNERWLVELN QEAMPRLRVN ATYAGMVRRA DSSADNTFMR NQLQEARWFI KSLQSRNETL 361 MKVATQIVEH QRGFLDYGEE AMKPLVLHDI AEAVGMHEST ISRVTTQKYM HTPRGIFELK 421 YFFSSHVSTA EGGECSSTAI RAIIKKLVAA ENAKKPLSDS KIAGLLEAQG IQVARRTVAK 481 YRESLGIAPS SERKRLV //