LOCUS ALR14730.1 157 aa PRT BCT 02-SEP-2016 DEFINITION Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 transcription elongation factor GreA protein. ACCESSION CP009977-265 PROTEIN_ID ALR14730.1 SOURCE Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 ORGANISM Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio. REFERENCE 1 (bases 1 to 3248023) AUTHORS Lee,H. and Church,G.M. TITLE Vibrio natriegens genome v1.0 JOURNAL Unpublished REFERENCE 2 (bases 1 to 3248023) AUTHORS Lee,H. and Church,G.M. TITLE Direct Submission JOURNAL Submitted (21-NOV-2014) Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 233, Boston, MA 02115, USA COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: RS HGAP v. 3 Assembly Name :: Vnat1.0 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 96.48x Sequencing Technology :: PacBio ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 11/24/2014 10:39:19 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 2.9 (rev. 452286) Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes :: 4,665 CDS :: 4,459 Pseudo Genes :: 42 rRNAs :: 34 (5S, 16S, 23S) tRNAs :: 129 ncRNA :: 1 Frameshifted Genes :: 10 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759" /mol_type="genomic DNA" /strain="ATCC 14048" /isolation_source="salt marsh mud" /culture_collection="ATCC:14048" /type_material="type strain of Vibrio natriegens" /db_xref="taxon:1219067" /chromosome="1" /country="USA" /collection_date="27-Feb-2012" protein /gene="greA" /locus_tag="PN96_01595" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_020333910.1" /note="necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus; Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MEKVPMTLRG EQMLREELDR LLKLRPQISA AIAEARELGD LKENAEYHAA REEQGICEAQ 61 IRDIEYKLSV AQVIDVTKME NTGKVIFGTT VTLIDVDTDE EKTYQIVGDD EADIKAGRIS 121 VSSPIARGLI GKMEGDEVVI ETPGGAKDFE IDRVEYL //